Tested Applications
| Positive WB detected in | rat liver tissue, mouse liver tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
27943-1-AP targets GPD1 in WB, ELISA applications and shows reactivity with Human, Mouse, Rat samples.
| Tested Reactivity | Human, Mouse, Rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag27703 Product name: Recombinant human GPD1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 40-181 aa of BC032234 Sequence: VFEEDIGGKKLTEIINTQHENVKYLPGHKLPPNVVAVPDVVQAAEDADILIFVVPHQFIGKICDQLKGHLKANATGISLIKGVDEGPNGLKLISEVIGERLGIPMSVLMGANIASEVADEKFCETTIGCKDPAQGQLLKELM Predict reactive species |
| Full Name | glycerol-3-phosphate dehydrogenase 1 (soluble) |
| Calculated Molecular Weight | 349 aa, 38 kDa |
| Observed Molecular Weight | 32-42 kDa |
| GenBank Accession Number | BC032234 |
| Gene Symbol | GPD1 |
| Gene ID (NCBI) | 2819 |
| RRID | AB_2881016 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P21695 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for GPD1 antibody 27943-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The GPD1 Polyclonal antibody (Cat# 27943-1-AP) has 2 total citations, both published in Molecular Cell (IF 16). The cited studies focus on metabolic research, specifically the uncoupled glycerol-3-phosphate shuttle in kidney cancer and its role in lipid synthesis, as well as saturation of mitochondrial NADH shuttles driving aerobic glycolysis in proliferating cells. Both papers used the antibody for Western blot in human and/or mouse samples.
| Species | Application | Title |
|---|---|---|
Mol Cell Saturation of the mitochondrial NADH shuttles drives aerobic glycolysis in proliferating cells | ||
Mol Cell Uncoupled glycerol-3-phosphate shuttle in kidney cancer reveals that cytosolic GPD is essential to support lipid synthesis
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