Tested Applications
| Positive WB detected in | HeLa cells, HepG2 cells |
| Positive IHC detected in | human ovary cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
28400-1-AP targets MSTO1 in WB, IHC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag29067 Product name: Recombinant human MSTO1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 28-256 aa of BC002535 Sequence: QDAALGRATDSKEPPGELCPDVLYRTGRTLHGQETYTPRLILMDLKGSLSSLKEEGGLYRDKQLDAAIAWQGKLTTHKEELYPKNPYLQDFLSAEGVLSSDGVWRVKSIPNGKGSSPLPTATTPKPLIPTEASIRVWSDFLRVHLHPRSICMIQKYNHDGEAGRLEAFGQGESVLKEPKYQEELEDRLHFYVEECDYLQGFQILCDLHDGFSGVGAKAAELLQDEYSGR Predict reactive species |
| Full Name | misato homolog 1 (Drosophila) |
| Observed Molecular Weight | 57-62 kDa |
| GenBank Accession Number | BC002535 |
| Gene Symbol | MSTO1 |
| Gene ID (NCBI) | 55154 |
| RRID | AB_2881133 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9BUK6 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
MSTO1, also named as LST005 and SLTP005, belongs to the misato family. Human MSTO1 is a ubiquitously expressed, evolutionary conserved protein with homologous regions to GTPases. MSTO1 is a cytoplasmic protein that modulates mitochondrial dynamics by promoting mitochondrial fusion. MSTO1 has 7 isoforms produced by alternative splicing. (PMID: 33612823)
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for MSTO1 antibody 28400-1-AP | Download protocol |
| WB protocol for MSTO1 antibody 28400-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The MSTO1 Polyclonal antibody (Cat# 28400-1-AP) has 1 citation. It appears in Cancer Gene Ther, where the study investigated how MSTO1 modulates RAD51 activity to safeguard mitochondrial DNA integrity and control immune responses. The research falls within the fields of mitochondrial biology, DNA repair, and immune regulation in a cancer context.
| Species | Application | Title |
|---|---|---|
Cancer Gene Ther MSTO1 modulates RAD51 activity to safeguard mitochondrial DNA integrity and control immune responses. |
