Tested Applications
| Positive WB detected in | K-562 cells, HEK-293 cells |
| Positive IHC detected in | human ovary cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
28437-1-AP targets KMT2C in WB, IHC, IF, CoIP, ChIP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag28875 Product name: Recombinant human KMT2C protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 2771-3000 aa of NM_170606 Sequence: SMFNEELDLPIDDKLDNQCVSVEPKKKEQENKTLVLSDKHSPQKKSTVTNEVKTEVLSPNSKVESKCETEKNDENKDNVDTPCSQASAHSDLNDGEKTSLHPCDPDLFEKRTNRETAGPSANVIQASTQLPAQDVINSCGITGSTPVLSSLLANEKSDNSDIRPSGSPPPPTLPASPSNHVSSLPPFIAPPGRVLDNAMNSNVTVVSRVNHVFSQGVQVNPGLIPGQSTV Predict reactive species |
| Full Name | myeloid/lymphoid or mixed-lineage leukemia 3 |
| Calculated Molecular Weight | 541 kDa |
| Observed Molecular Weight | 541kDa, 269 kDa |
| GenBank Accession Number | NM_170606 |
| Gene Symbol | KMT2C |
| Gene ID (NCBI) | 58508 |
| RRID | AB_2918165 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8NEZ4 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Lysine (K)-specific methyltransferase 2C (KMT2C, also known as MLL3) belongs to the mixed-lineage leukemia (MLL) family of histone methyltransferases which methylate the histone 3 tail at lysine 4 (H3K4) as part of the complex proteins associated with Set 1 (COMPASS) complex. Although originally identified as oncogenic fusions in leukemia, genome-wide mutation studies have revealed frequent, presumably loss-of-function, mutations in various members of the MLL family, including MLL2/KMT2B, MLL3/KMT2C, and MLL4/KMT2D in a variety of malignancies, particularly solid tumors. Mechanistic studies of KMT2C in normal cells have focused primarily on its role in enhancer regulation by deposition of H3K4me1 marks.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for KMT2C antibody 28437-1-AP | Download protocol |
| WB protocol for KMT2C antibody 28437-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The KMT2C polyclonal antibody (Cat# 28437-1-AP) has 7 citations published in journals including Molecular Cell, Nature Chemical Biology, Advanced Science, Noncoding RNA Research, International Immunopharmacology, Journal of Biological Chemistry, and BMC Medical Genomics. The associated research fields span epigenetics, cancer biology (prostate, esophageal, and renal cell carcinoma), DNA replication stress, autophagy, intervertebral disc degeneration, viral-induced inflammation, and genetic disorders.
| Species | Application | Title |
|---|---|---|
Mol Cell Isoform-specific function of NSD3 in DNA replication stress confers resistance to PARP inhibitors in prostate cancer. | ||
Nat Chem Biol UFL1 triggers replication fork degradation by MRE11 in BRCA1/2-deficient cells | ||
Adv Sci (Weinh) TAOK3 Facilitates Esophageal Squamous Cell Carcinoma Progression and Cisplatin Resistance Through Augmenting Autophagy Mediated by IRGM | ||
Int Immunopharmacol Polysaccharides from Platycodonis Radix ameliorated respiratory syncytial virus-induced epithelial cell apoptosis and inflammation through activation of miR-181a-mediated Hippo and SIRT1 pathways. | ||
Noncoding RNA Res Epigenetic H3K4me3 activation of miR-155-5p promotes intervertebral disc degeneration via autophagy and ageing in nucleus pulposus cells.
| ||
J Biol Chem TRIM28 represses renal cell carcinoma cell proliferation by inhibiting TFE3/KDM6A-regulated autophagy |
