Tested Applications
| Positive WB detected in | A549 cells, NCI-H1299 cells, PC-3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
28594-1-AP targets Siglec-15 in WB, ELISA applications and shows reactivity with Human samples.
| Tested Reactivity | Human |
| Cited Reactivity | human, mouse, pig |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag29829 Product name: Recombinant human SIGLEC15 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 20-160 aa of NM_213602 Sequence: FVRTKIDTTENLLNTEVHSSPAQRWSMQVPPEVSAEAGDAAVLPCTFTHPHRHYDGPLTAIWRAGEPYAGPQVFRCAAARGSELCQTALSLHGRFRLLGNPRRNDLSLRVERLALADDRRYFCRVEFAGDVHDRYESRHGV Predict reactive species |
| Full Name | sialic acid binding Ig-like lectin 15 |
| Calculated Molecular Weight | 36 kDa |
| Observed Molecular Weight | 38-40 kDa |
| GenBank Accession Number | NM_213602 |
| Gene Symbol | Siglec-15 |
| Gene ID (NCBI) | 284266 |
| RRID | AB_2918178 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q6ZMC9 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Sialic acid binding Ig-like lectin 15 (Siglec-15) is a member of the Siglec family of glycan-recognition proteins. Siglec-15 is a type-I transmembrane protein consisting of two immunoglobulin (Ig)-like domains, a transmembrane domain, and a short cytoplasmic tail (PMID: 17483134). It is mainly expressed on a subset of myeloid lineage cells and can be upregulated in many cancers (PMID: 32939323). Siglec-15 has been reported as a immune suppressor and a potential target for normalization cancer immunotherapy (PMID: 30833750). Siglec-15 has a calculated molecular weight of 36 kDa. It has been detected at 38-40 kDa due to glycosylation (PMID: 32921411).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Siglec-15 antibody 28594-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The Siglec-15 Polyclonal antibody (Cat# 28594-1-AP) has 5 total citations. These appear in Genes Dis, Cell Mol Life Sci, DNA Cell Biol, J Oral Pathol Med, and Transl Oncol. The research fields span triple-negative breast cancer immunosuppression, porcine epidemic diarrhea virus infection, cisplatin resistance in cervical cancer, head and neck squamous cell carcinoma prognosis, and TNBC–macrophage crosstalk via the NF-κB/STAT3 axis.
| Species | Application | Title |
|---|---|---|
Genes Dis SIGLEC15 modulates the immunosuppressive microenvironment and suppresses malignant phenotypes in triple-negative breast cancer.
| ||
Cell Mol Life Sci Siglec-15 is a putative receptor for porcine epidemic diarrhea virus infection | ||
DNA Cell Biol Siglec-15 Enhances Cisplatin Resistance in Cervical Cancer Cells by Promoting Mitochondrial Retrograde Signaling Through Activation of the MAPK Pathway. | ||
J Oral Pathol Med AARS1 Implicates Malignancy and Immune Infiltration in Head and Neck Squamous Cell Carcinoma, Serving as a Prognostic Predictor | ||
Transl Oncol Siglec-15 promotes a feed-forward loop between TNBC cells and macrophages via the NF-κB/DcR3/CCL18/STAT3 axis. |
Reviews
What customers say
One reviewer used this antibody at a 1:500 dilution for immunofluorescence on prostate cancer FFPE tissue and reported positive staining with slight background. The review was rated 4 out of 5 stars. Overall, the feedback indicates reliable performance in FFPE tissue applications with good specificity, though minor background was noted.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Rebecca (Verified Customer) (07-21-2022) | Positive staining identified at 1:500 dilution in patient FFPE tissue. Slight background.
 |
