Tested Applications
| Positive WB detected in | HEK-293 cells, THP-1 cells, mouse kidney tissue, rat kidney tissue, SMMC-7721 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
29329-1-AP targets GPX1 in WB, IHC, IF, ELISA applications and shows reactivity with Human, Mouse, Rat samples.
| Tested Reactivity | Human, Mouse, Rat |
| Cited Reactivity | human, mouse, rat, pig, chicken, bovine, sheep |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag31101 Product name: Recombinant human GPX1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 102-203 aa of BC000742 Sequence: GGGFEPNFMLFEKCEVNGAGAHPLFAFLREALPAPSDDATALMTDPKLITWSPVCRNDVAWNFEKFLVGPDGVPLRRYSRRFQTIDIEPDIEALLSQGPSCA Predict reactive species |
| Full Name | glutathione peroxidase 1 |
| Calculated Molecular Weight | 22 kDa |
| Observed Molecular Weight | 22 kDa |
| GenBank Accession Number | BC000742 |
| Gene Symbol | GPX1 |
| Gene ID (NCBI) | 2876 |
| RRID | AB_2918283 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P07203 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Gpx protein, known as selenoprotein, consists selenium in its catalytic site. Gpx1 is an abundant isoform, which involves in scavenging endogenous ROS using electron provided by reduced glutathione and ubiquitously expressed the cytoplasm and mitochondria of all cell types. (PMID: 30632027)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for GPX1 antibody 29329-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
Anti-GPX1 antibody (Cat# 29329-1-AP) has accumulated 50 citations across journals including Cell, Nature Neuroscience, Nature Communications, Molecular Cell, Redox Biology, Free Radical Biology and Medicine, and Cell Death Discovery. Research fields span ferroptosis, oxidative stress, cancer biology, neurodegeneration, cardiovascular disease, diabetic nephropathy, liver injury, pulmonary fibrosis, reproductive toxicity, and mitochondrial homeostasis, with primary applications in Western blotting, immunofluorescence, and immunohistochemistry.
| Species | Application | Title |
|---|---|---|
Cell A GPX1-OSBPL8 axis mediates noncanonical in vivo ferroptosis and cancer growth suppression.
| ||
Bioact Mater Mesenchymal stromal/stem cell spheroid-derived extracellular vesicles advance the therapeutic efficacy of 3D-printed vascularized artificial liver lobules in liver failure treatment | ||
Nat Neurosci Derivation and transcriptional reprogramming of border-forming wound repair astrocytes after spinal cord injury or stroke in mice | ||
Nat Commun Plant-derived hydrogel and photosynthetic nano-units for myocardial infarction therapy | ||
Redox Biol Iron(III)-salophene catalyzes redox cycles that induce phospholipid peroxidation and deplete cancer cells of ferroptosis-protecting cofactors |
