Tested Applications
| Positive WB detected in | HCT 116 cells, HepG2 cells, MCF-7 cells, MDA-MB-231 cells, rat kidney tissue, mouse kidney tissue |
| Positive IHC detected in | human placenta tissue, rat kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells, A549 cells |
| Positive FC (Intra) detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 ul suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
29771-1-AP targets EBP50/NHERF-1 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag30878 Product name: Recombinant human SLC9A3R1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 260-358 aa of NM_004252 Sequence: TNGEIQKENSREALAEAALESPRPALVRSASSDTSEELNSQDSPPKQDSTAPSSTSSSDPILDFNISLAMAKERAHQKRSSKRAPQMDWSKKNELFSNL Predict reactive species |
| Full Name | solute carrier family 9 (sodium/hydrogen exchanger), member 3 regulator 1 |
| Calculated Molecular Weight | 39 kDa |
| Observed Molecular Weight | 50 kDa |
| GenBank Accession Number | NM_004252 |
| Gene Symbol | SLC9A3R1/EBP50/NHERF-1 |
| Gene ID (NCBI) | 9368 |
| RRID | AB_2935478 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O14745 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Na+/H+ exchanger regulatory factor 1 (NHERF-1, also known as EBP50 ezrin-binding protein of 50 kDa, SLCA9A3R1) is a phosphoprotein that assembles multiprotein complexes via two PDZ domains and a C-terminal ezrin-binding domain. NHERF-1 was initially recognized as a scaffolding protein that recruits membrane transporters/receptors and cytoplasmic signaling proteins into functional complexes localized at or near the plasma membrane in epithelial cells (PMID: 19308292).
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for EBP50/NHERF-1 antibody 29771-1-AP | Download protocol |
| IF protocol for EBP50/NHERF-1 antibody 29771-1-AP | Download protocol |
| IHC protocol for EBP50/NHERF-1 antibody 29771-1-AP | Download protocol |
| WB protocol for EBP50/NHERF-1 antibody 29771-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
EBP50/NHERF-1 Polyclonal antibody (catalog 29771-1-AP) has 2 total citations. Citations appear in two journals: Cell Death Dis and Int J Mol Sci. Associated research fields include prostate cancer progression (OPRK1 driving SLC9A3R1 progression to neuroendocrine prostate cancer) and reproductive biology (effects of acrylamide on mouse implantation and decidualization).
| Species | Application | Title |
|---|---|---|
