Tested Applications
| Positive WB detected in | A431 cells, U2OS cells, human placenta tissue, mouse ovary tissue |
| Positive IHC detected in | human lung cancer tissue, human stomach cancer tissue, mouse kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
29834-1-AP targets RPS23 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag31190 Product name: Recombinant human RPS23 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 2-143 aa of NM_001025 Sequence: GKCRGLRTARKLRSHRRDQKWHDKQYKKAHLGTALKANPFGGASHAKGIVLEKVGVEAKQPNSAIRKCVRVQLIKNGKKITAFVPNDGCLNFIEENDEVLVAGFGRKGHAVGDIPGVRFKVVKVANVSLLALYKGKKERPRS Predict reactive species |
| Full Name | ribosomal protein S23 |
| Calculated Molecular Weight | 16 kDa |
| Observed Molecular Weight | 16-18 kDa |
| GenBank Accession Number | NM_001025 |
| Gene Symbol | RPS23 |
| Gene ID (NCBI) | 6228 |
| RRID | AB_3669698 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P62266 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
RPS23 (40S ribosomal protein S23), also called small ribosomal subunit protein uS12, is a subunit of the 40S ribosome and the first precursor of the small eukaryotic ribosomal subunit (PMID: 34516797). It is positioned in the decoding center of the ribosome that serves to maintain translational fidelity by monitoring the complementarity between the mRNA codons being translated and the anti-codons of aminoacyl-tRNAs (PMID: 28257692; 23636399).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for RPS23 antibody 29834-1-AP | Download protocol |
| IHC protocol for RPS23 antibody 29834-1-AP | Download protocol |
| WB protocol for RPS23 antibody 29834-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The RPS23 Polyclonal antibody (Cat# 29834-1-AP) has 1 total citation. It was cited in Journal of Translational Medicine (IF 9.7), in a 2025 study investigating how paternal ribosome biogenesis dysfunction induced by cyclophosphamide-busulfan chemotherapy contributes to early embryonic developmental impairment in mice. The associated research fields include reproductive biology, ribosome biogenesis, and developmental toxicology. The antibody was used for Western blot in mouse samples.
| Species | Application | Title |
|---|---|---|
J Transl Med Paternal ribosome biogenesis dysfunction caused by cyclophosphamide-busulfan chemotherapy contributes to early embryonic developmental impairment in mice. |
