Tested Applications
| Positive WB detected in | HeLa cells, MOLT-4 cells, PC-3 cells, Raji cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
85444-3-RR targets PFKFB4 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | bovine |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag30571 Product name: Recombinant human PFKFB4 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 139-232 aa of NM_001317134 Sequence: ATIFNFGEQNGYKTFFVESICVDPEVIAANIVQVKLGSPDYVNRDSDEATEDFMRRIECYENSYESLDEDLDRDLSYIKIMDVGQSYVVNRVAD Predict reactive species |
| Full Name | 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 4 |
| Calculated Molecular Weight | 54 kDa |
| Observed Molecular Weight | 54 kDa |
| GenBank Accession Number | NM_001317134 |
| Gene Symbol | PFKFB4 |
| Gene ID (NCBI) | 5210 |
| RRID | AB_3744218 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q16877 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
PFKFB4 (6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 4), is a bi-function enzyme. On one hand, PFKFB4 synthesizes fructose 2,6-bisphosphate (F2,6-BP) to stimulate glycolysis, playing important roles in metabolic processes. On the other hand, PFKFB4 acts as a phosphatase to hydrolyze F2,6-BP into fructose-6-phosphate (F6P) and inorganic phosphate (Pi), functioning as a protein kinase to phosphorylate protein substrates. The dual function of PFKFB4 makes it involved in multiple biological processes such as cell cycle regulation, cell proliferation and transcriptional regulation(PMID: 33593309). PFKFB4 has been reported to be involved in a variety of cancers including human bladder cancer, gastric cancer, colon cancer, breast cancer and other malignant tumors(PMID: 21396842, PMID: 33732372, PMID: 31244553).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for PFKFB4 antibody 85444-3-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The recombinant monoclonal antibody PFKFB4 (catalog 85444-3-RR) has 1 citation in the journal Antioxidants (Basel) (Impact Factor 8.2). The cited study focuses on proteomic identification of PFKFB3 and PFKFB4 in dairy cows with clinical mastitis, associated with coenzyme metabolism and redox imbalance. The antibody was used in IHC and IF applications in a bovine sample context.
| Species | Application | Title |
|---|---|---|
Antioxidants (Basel) Proteomic Identification of PFKFB3 and PFKFB4 Associated with Coenzyme Metabolism and Redox Imbalance in Dairy Cows with Clinical Mastitis. |
