PFKFB4 Recombinant monoclonal antibody, PBS Only (Capture)

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• PFKFB4 Recombinant monoclonal antibody, PBS Only (Capture) (85444-3-PBS)

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  WB analysis using 85444-3-RR (same clone as 85444-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85444-3-RR (PFKFB4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85444-3-PBS in a different storage buffer formulation.

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  Sandwich ELISA sample test of MP01998-1

  The mean PFKFB4 concentration was determined to be 167.95 ng/mL in HepG2 cell extract based on a 1.8 mg/mL extract load.

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  Sandwich ELISA standard curve of MP01998-1

  Sandwich ELISA standard curve of MP01998-1, Human PFKFB4 Recombinant Matched Antibody Pair - PBS only. 85444-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag30571. 85444-2-PBS was HRP conjugated as the detection antibody. Range: 0.781-25 ng/mL

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  Cytometric bead array standard curve of MP01998-1

  Cytometric bead array standard curve of MP01998-1, PFKFB4 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 85444-3-PBS. Detection antibody: 85444-2-PBS. Standard: Ag30571. Range: 0.781-25 ng/mL.

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  Affinity and Kinetic Characterization of 85444-3-RR

  Biolayer interferometry (BLl) kinetic assays of 85444-3-RR against Human PFKFB4 were performed. The affinity constant is 0.705 nM.

PFKFB4 Uni-rAb^® Recombinant Antibody for WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA

Cat No. 85444-3-PBS

Clone No.242962H8

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA

Synonyms

242962H8, 6PF 2 K/Fru 2,6 P2ase 4, PFK/FBPase 4

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Product Information

85444-3-PBS targets PFKFB4 as part of a matched antibody pair:

MP01998-1: 85444-3-PBS capture and 85444-2-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag30571

  Product name: Recombinant human PFKFB4 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 139-232 aa of NM_001317134

  Sequence: ATIFNFGEQNGYKTFFVESICVDPEVIAANIVQVKLGSPDYVNRDSDEATEDFMRRIECYENSYESLDEDLDRDLSYIKIMDVGQSYVVNRVAD

• Full Name: 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 4
• Calculated Molecular Weight: 54 kDa
• Observed Molecular Weight: 54 kDa
• GenBank Accession Number: NM_001317134
• Gene Symbol: PFKFB4
• Gene ID (NCBI): 5210
• RRID: AB_3744218
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q16877
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

PFKFB4 (6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 4), is a bi-function enzyme. On one hand, PFKFB4 synthesizes fructose 2,6-bisphosphate (F2,6-BP) to stimulate glycolysis, playing important roles in metabolic processes. On the other hand, PFKFB4 acts as a phosphatase to hydrolyze F2,6-BP into fructose-6-phosphate (F6P) and inorganic phosphate (Pi), functioning as a protein kinase to phosphorylate protein substrates. The dual function of PFKFB4 makes it involved in multiple biological processes such as cell cycle regulation, cell proliferation and transcriptional regulation(PMID: 33593309). PFKFB4 has been reported to be involved in a variety of cancers including human bladder cancer, gastric cancer, colon cancer, breast cancer and other malignant tumors(PMID: 21396842, PMID: 33732372, PMID: 31244553).