Tested Applications
| Positive WB detected in | HEK-293 cells, HepG2 cells, HeLa cells, Jurkat cells, K-562 cells |
| Positive IP detected in | HEK-293 cells |
| Positive IHC detected in | human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:150-1:600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
30141-1-AP targets KIF13B in WB, IHC, IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag30876 Product name: Recombinant human KIF13B protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1648-1826 aa of NM_015254 Sequence: VRRVRASELRSFSRMLAGDPGCSPGAEGNAPAPGAGGQALASDSEEADEVPEWLREGEFVTVGAHKTGVVRYVGPADFQEGTWVGVELDLPSGKNDGSIGGKQYFRCNPGYGLLVRPSRVRRATGPVRRRSTGLRLGAPEARRSATLSGSATNLASLTAALAKADRSHKNPENRKSWAS Predict reactive species |
| Full Name | kinesin family member 13B |
| Calculated Molecular Weight | 203 kDa |
| Observed Molecular Weight | 250 kDa |
| GenBank Accession Number | NM_015254 |
| Gene Symbol | KIF13B |
| Gene ID (NCBI) | 23303 |
| RRID | AB_3086245 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9NQT8 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
KIF13B, Also known as GAKIN, is a 250-kD phosphoprotein. KIF13B contains a consensus ATP/GTP-binding motif within its N-terminal motor domain; a stalk domain containing several short alpha-helical segments; and a CAP-gly motif in its C terminus characteristic of microtubule-based cytoskeleton components(PMID: 10859302).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for KIF13B antibody 30141-1-AP | Download protocol |
| IP protocol for KIF13B antibody 30141-1-AP | Download protocol |
| WB protocol for KIF13B antibody 30141-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The KIF13B Polyclonal antibody (Cat# 30141-1-AP) has 1 total citation. It appears in the American Journal of Physiology – Cell Physiology (Impact Factor 5.4), published in 2025. The cited study investigates how PCP protein Prickle 1 regulates Sertoli cell and testis function through cytoskeletal organization, placing the research in the fields of reproductive biology and cytoskeletal regulation. The antibody was applied for Western blotting using rat samples.
| Species | Application | Title |
|---|---|---|
Am J Physiol Cell Physiol PCP protein Prickle 1 regulates Sertoli cell and testis function via cytoskeletal organization through the recruitment of multiple regulatory proteins |
