Tested Applications
| Positive WB detected in | mouse brain tissue, mouse heart tissue, mouse liver tissue, mouse pancreas tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
32928-1-AP targets CIR in WB, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag38647 Product name: Recombinant human CIR protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-160 aa of NM_004882.3 Sequence: MGKSFANFMCKKDFHPASKSNIKKVWMAEQKISYDKKKQEELMQQYLKEQESYDNRLLMGDERVKNGLNFMYEAPPGAKKENKEKEETEGETEYKFEWQKGAPREKYAKDDMNIRDQPFGIQVRNVRCIKCHKWGHVNTDRECPLFGLSGINASSVPTDG Predict reactive species |
| Full Name | CBF1 interacting corepressor |
| Calculated Molecular Weight | 52kDa，450aa |
| Observed Molecular Weight | 23 kDa |
| GenBank Accession Number | NM_004882.3 |
| Gene Symbol | CIR |
| Gene ID (NCBI) | 9541 |
| RRID | AB_3742793 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | Q86X95 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CIR, a protein originally identified as a CBF1-interacting protein and reported to act as a transcriptional corepressor. CIR is a member of the family of SR-related proteins and that CIR plays a role in splicing regulation. In addition to a basic lysine and acidic serine-rich (BA) domain and a zinc knuckle-like motif, CIR has an arginine/serine dipeptide repeat (RS) domain in its C terminal region. The RS domain has been reported to be present in the superfamily of SR proteins, which are involved in splicing reactions (PMID: 15652350).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CIR antibody 32928-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Reviews
What customers say
The single review for this product is a 5-star rating. The user reports a strong signal by Western Blot at a 1:1000 dilution in B cells. Although the antibody produced two bands, both decreased as expected upon treatment, which validated their omics results. They also noted using a blotting accelerator for imaging. Overall, the reviewer was satisfied with the antibody's performance and its ability to confirm their experimental findings.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Annabel (Verified Customer) (12-23-2025) | Strong signal but produces two bands by western blot. However, both go down as expected with treatment, validating our omics results. Imaged using blotting accelerator
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