Tested Applications
| Positive WB detected in | HepG2 cells, HeLa cells, Jurkat cells, L02 cells, PC-3 cells, SK-BR-3 cell |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human cervical cancer tissue, human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
51066-1-AP targets IkB Alpha in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha |
| Calculated Molecular Weight | 36 kDa |
| Observed Molecular Weight | 39 kDa |
| GenBank Accession Number | BC002601 |
| Gene Symbol | IkB Alpha |
| Gene ID (NCBI) | 4792 |
| RRID | AB_2235946 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P25963 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha (NFKBIA, synonyms: IKBA, MAD-3, NFKBI). NFKB1 or NFKB2 is bound to REL, RELA , or RELB to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA or NFKBIB), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, or IKBKB) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for IkB Alpha antibody 51066-1-AP | Download protocol |
| IP protocol for IkB Alpha antibody 51066-1-AP | Download protocol |
| WB protocol for IkB Alpha antibody 51066-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
IκB Alpha Polyclonal Antibody (Cat# 51066-1-AP) has accumulated 22 citations across journals including Journal of Hazardous Materials, International Journal of Molecular Medicine, Molecular Medicine, Frontiers in Immunology, Journal of Ethnopharmacology, Life Sciences, Nutrients, International Immunopharmacology, Scientific Reports, FASEB Journal, Journal of Immunology, PLoS ONE, and Biochemical Pharmacology. Associated research fields include NF-κB signaling, inflammation, osteoarthritis, osteoclastogenesis, liver disease, neuroinflammation, colitis, pneumonia, and cancer.
| Species | Application | Title |
|---|---|---|
J Hazard Mater Melatonin alleviates Ochratoxin A-induced liver inflammation involved intestinal microbiota homeostasis and microbiota-independent manner. | ||
Int J Mol Med Dihydroartemisinin attenuates osteoclast formation and bone resorption via inhibiting the NF‑κB, MAPK and NFATc1 signaling pathways and alleviates osteoarthritis | ||
Mol Med Age-related decline in melatonin contributes to enhanced osteoclastogenesis via disruption of redox homeostasis | ||
Front Immunol Aspartate Metabolism Facilitates IL-1β Production in Inflammatory Macrophages. | ||
J Ethnopharmacol Dendrobium huoshanense polysaccharides alleviate DSS-induced ulcerative colitis in mice: comparison between different parts of plant and role of GLP-1/GLP-1R axis. |
