IkB Alpha Polyclonal antibody, PBS Only

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  WB analysis of PC-3 using 51066-1-AP (same clone as 51066-1-PBS)

  PC-3 cells were subjected to SDS PAGE followed by western blot with 51066-1-AP (IkB alpha antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 51066-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 51066-1-AP (same clone as 51066-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 51066-1-AP (IkB alpha antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 51066-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 51066-1-AP (same clone as 51066-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 51066-1-AP (IkB alpha antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 51066-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 51066-1-AP (same clone as 51066-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 51066-1-AP (IkB alpha antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 51066-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Jurkat using 51066-1-AP (same clone as 51066-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 51066-1-AP (IkB alpha antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 51066-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 51066-1-AP (same clone as 51066-1-PBS)

  IP result of anti-IkB Alpha (IP:51066-1-AP, 4ug; Detection:51066-1-AP 1:1000) with HeLa cells lysate 3200ug. This data was developed using the same antibody clone with 51066-1-PBS in a different storage buffer formulation.

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  IHC staining of human cervical cancer using 51066-1-AP (same clone as 51066-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 51066-1-AP (IkB alpha antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 51066-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human cervical cancer using 51066-1-AP (same clone as 51066-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 51066-1-AP (IkB alpha antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 51066-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 51066-1-AP (same clone as 51066-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver using 51066-1-AP (IkB alpha antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 51066-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human cervical cancer using 51066-1-AP (same clone as 51066-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 51066-1-AP (IkB alpha antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 51066-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human cervical cancer using 51066-1-AP (same clone as 51066-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 51066-1-AP (IkB alpha antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 51066-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 51066-1-AP (same clone as 51066-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver using 51066-1-AP (IkB alpha antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 51066-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 51066-1-AP (same clone as 51066-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver using 51066-1-AP (IkB alpha antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 51066-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 51066-1-AP (same clone as 51066-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver using 51066-1-AP (IkB alpha antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 51066-1-PBS in a different storage buffer formulation.

IkB Alpha Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Cat No. 51066-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

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Product Information

51066-1-PBS targets IkB Alpha in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha
• Calculated Molecular Weight: 36 kDa
• Observed Molecular Weight: 39 kDa
• GenBank Accession Number: BC002601
• Gene Symbol: IkB Alpha
• Gene ID (NCBI): 4792
• RRID: AB_2235946
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P25963
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha (NFKBIA, synonyms: IKBA, MAD-3, NFKBI). NFKB1 or NFKB2 is bound to REL, RELA , or RELB to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA or NFKBIB), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, or IKBKB) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime.