Tested Applications
| Positive WB detected in | mouse liver tissue, rat liver tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
55091-1-AP targets PTCH2 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | patched homolog 2 (Drosophila) |
| Calculated Molecular Weight | 131 kDa |
| Observed Molecular Weight | 131-140 kDa |
| GenBank Accession Number | NM_003738 |
| Gene Symbol | PTCH2 |
| Gene ID (NCBI) | 8643 |
| RRID | AB_10858392 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9Y6C5 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
PTCH1 (patched homolog 1) and PTCH2 are twelve-pass transmembrane proteins that act receptors of Hedgehog signaling pathway (PMID: 8906794; 9811851). Signals of Hedgehog family proteins (SHH, IHH and DHH in humans) are transduced through Patched family receptors (PTCH1 and PTCH2) and Smoothened (SMO) to GLI family transcription factors. Hedgehog pathway plays a critical role in embryonic development and tissue homeostasis, and its dysregulation has been implicated in many human diseases including congenital disorder and cancer (PMID: 23719536; 23532857). PTCH2 may function as a tumor suppressor in the Hedgehog signaling pathway. Alterations in PTCH2 gene have been associated with nevoid basal cell carcinoma syndrome, basal cell carcinoma, medulloblastoma, and susceptibility to congenital macrostomia. This antibody is specific to PTCH2.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for PTCH2 antibody 55091-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The PTCH2 Polyclonal antibody (Cat# 55091-1-AP) has 3 total citations. These appear in Cell Research, Experimental & Molecular Medicine, and iScience. The associated research fields include breast cancer bone metastasis (immune-suppressive osteoblastic niches), spinal cord injury recovery via Hedgehog signaling pathway activation, and progression from chronic pancreatitis to pancreatic intraepithelial neoplasia. All citations used the antibody for Western blot in mouse, rat, or human samples.
| Species | Application | Title |
|---|---|---|
Cell Res SCUBE2 mediates bone metastasis of luminal breast cancer by modulating immune-suppressive osteoblastic niches | ||
Exp Mol Med Lentivirus-mediated silencing of the PTC1 and PTC2 genes promotes recovery from spinal cord injury by activating the Hedgehog signaling pathway in a rat model. | ||
iScience 4-(Methylnitrosamino)-1-(3-pyridyl)-1-butanone provokes progression from chronic pancreatitis to pancreatic intraepithelial neoplasia. |
