Tested Applications
| Positive WB detected in | HEK-293 cells, HeLa cells, HepG2 cells, Jurkat cells, SGC-7901cells, NIH/3T3 cells |
| Positive IP detected in | HepG2 cells |
| Positive IHC detected in | human stomach cancer tissue, human colon cancer tissue, human kidney tissue, mouse liver tissue, rat kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
55309-1-AP targets MAT2A in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, pig |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | methionine adenosyltransferase II, alpha |
| Calculated Molecular Weight | 44 kDa |
| Observed Molecular Weight | 47 kDa, 49 kDa |
| GenBank Accession Number | NM_005911 |
| Gene Symbol | MAT2A |
| Gene ID (NCBI) | 4144 |
| RRID | AB_2881303 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P31153 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
MAT2A, also named as AMS2, MATA2 and AdoMet synthase 2, belongs to the AdoMet synthase family. It catalyzes the formation of S-adenosylmethionine from methionine and ATP. This antibody is specific to MATA2.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for MAT2A antibody 55309-1-AP | Download protocol |
| IHC protocol for MAT2A antibody 55309-1-AP | Download protocol |
| IP protocol for MAT2A antibody 55309-1-AP | Download protocol |
| WB protocol for MAT2A antibody 55309-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The MAT2A antibody (Cat# 55309-1-AP) has 31 total citations published across 26 journals, including Nature, Science, Cell Metabolism, Nature Metabolism, Nature Communications, Molecular Cell, and Advanced Science. Associated research fields span methionine metabolism, mTORC1 signaling, m6A RNA modification, ferroptosis, cancer biology (glioblastoma, bladder/gastric/osteosarcoma), obesity, liver disease, inflammation, viral replication, and bone biology.
| Species | Application | Title |
|---|---|---|
Cell Metab PRMT1 orchestrates with SAMTOR to govern mTORC1 methionine sensing via Arg-methylation of NPRL2 | ||
Nat Metab The SESAME complex regulates cell senescence through the generation of acetyl-CoA.
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