Tested Applications
| Positive WB detected in | Jurkat cells, U2OS cells, K-562 cells |
| Positive IP detected in | Jurkat cells |
| Positive IHC detected in | human colon cancer tissue, human oesophagus cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
55377-1-AP targets SETD2 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | SET domain containing 2 |
| Calculated Molecular Weight | 288 kDa |
| Observed Molecular Weight | 287 kDa |
| GenBank Accession Number | NM_014159 |
| Gene Symbol | SETD2 |
| Gene ID (NCBI) | 29072 |
| RRID | AB_2881318 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9BYW2 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
SETD2, also named as HIF1, HYPB, KIAA1732, KMT3A, SET2, p231HBP, hSET2 and HIP-1, belongs to the histone-lysine methyltransferase family and SET2 subfamily. It is a histone methyltransferase that methylates 'Lys-36' of histone H3. SETD2 probably plays a role in chromatin structure modulation during elongation via its interaction with hyperphosphorylated POLR2A. It binds DNA at promoters. SETD2 may also act as a transcription activator that binds to promoters.(PMID:16118227) It is a potential tumor suppressor gene in breast cancer.(PMID:20944102) This antibody is specific to SETD2.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for SETD2 antibody 55377-1-AP | Download protocol |
| IHC protocol for SETD2 antibody 55377-1-AP | Download protocol |
| IP protocol for SETD2 antibody 55377-1-AP | Download protocol |
| WB protocol for SETD2 antibody 55377-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The SETD2 antibody (Cat# 55377-1-AP) has 11 total citations across journals including Nature Communications, Nucleic Acids Research, Journal of Experimental & Clinical Cancer Research, Advanced Science, Cell Death & Disease, Phytotherapy Research, Cell Molecular Life Sciences, Function, Carcinogenesis, and Cell Reports. Research fields span epigenetics and chromatin regulation, cancer biology (renal, hepatocellular, cervical), ferroptosis, histone methylation, m⁶A RNA modification, ubiquitination, and pulmonary hypertension.
| Species | Application | Title |
|---|---|---|
Nat Commun Epigenomic modifications define chromatin states to regulate cell-free DNA fragmentomics. | ||
Nucleic Acids Res PHD finger protein 1 (PHF1) is a novel reader for histone H4R3 symmetric dimethylation and coordinates with PRMT5-WDR77/CRL4B complex to promote tumorigenesis.
| ||
J Exp Clin Cancer Res A novel FBW7/NFAT1 axis regulates cancer immunity in sunitinib-resistant renal cancer by inducing PD-L1 expression. | ||
Adv Sci (Weinh) H3K36me3-Guided m6A Modification of Oncogenic L1CAM-AS1 Drives Macrophage Polarization and Immunotherapy Resistance in Hepatocellular Carcinoma | ||
Cell Death Dis Knockdown of SETD2 promotes erastin-induced ferroptosis in ccRCC
| ||
Phytother Res Polydatin Alleviated Ischemia-Reperfusion Induced Kidney Injury by Inhibiting SETD2-Mediated Ferroptosis.
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Reviews
What customers say
One customer (Gaurav Kumar) rated this product 5/5 stars for Western Blot on HEK293 cells using a 1:1000 dilution (Lot 00044496). The reviewer noted that it works well with low protein quantity. Overall, the single review is positive, highlighting the antibody's sensitivity at low input levels.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Gaurav (Verified Customer) (02-27-2026) | Works with low protein quantity
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