Tested Applications
| Positive WB detected in | SMMC-7721 cells, A549 cells, HeLa cells, HepG2 cells, L02 cells, rat liver tissue |
| Positive IHC detected in | human liver cancer tissue, human colon cancer tissue, human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:5000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
60064-1-Ig targets HDGF in WB, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag1893 Product name: Recombinant human HDGF protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-240 aa of BC018991 Sequence: MSRSNRQKEYKCGDLVFAKMKGYPHWPARIDEMPEAAVKSTANKYQVFFFGTHETAFLGPKDLFPYEESKEKFGKPNKRKGFSEGLWEIENNPTVKASGYQSSQKKSCVEEPEPEPEAAEGDGDKKGNAEGSSDEEGKLVIDEPAKEKNEKGALKRRAGDLLEDSPKRPKEAENPEGEEKEAATLEVERPLPMEVEKNSTPSEPGSGRGPPQEEEEEEDEEEEATKEDAEAPGIRDHESL Predict reactive species |
| Full Name | hepatoma-derived growth factor (high-mobility group protein 1-like) |
| Calculated Molecular Weight | 26 kDa |
| Observed Molecular Weight | 40 kDa |
| GenBank Accession Number | BC018991 |
| Gene Symbol | HDGF |
| Gene ID (NCBI) | 3068 |
| RRID | AB_2117098 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P51858 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
HDGF, also named as HMG1L2, belongs to the HDGF family. It is a heparin-binding protein, with mitogenic activity for fibroblasts. HDGF acts as a transcriptional repressor. It plays important roles in the development and progression of cancers. HDGF may have a synergistic effect with VEGF during cancer development and Progression. (PMID:20848225)
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for HDGF antibody 60064-1-Ig | Download protocol |
| WB protocol for HDGF antibody 60064-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The anti-HDGF antibody (Cat# 60064-1-Ig) has 9 citations in journals including Nature Biomedical Engineering, Nature Cell Biology, Frontiers in Cell and Developmental Biology, Medical Oncology, Journal of Biomedical Research, Neural Plasticity, Comparative Biochemistry and Physiology D, Biochemical and Biophysical Research Communications, and Molecular Vision. Research fields span bladder cancer, hepatocellular carcinoma, CNS lymphoma, diabetic retinopathy, neuropathy, esophageal cancer, angiogenesis, and RNA modification biology.
| Species | Application | Title |
|---|---|---|
Nat Biomed Eng RModBlock antisense oligonucleotides as a universal tool for precise and efficient inhibition of RNA modifications. | ||
Nat Cell Biol 5-methylcytosine promotes pathogenesis of bladder cancer through stabilizing mRNAs. | ||
Front Cell Dev Biol NAP1L1 Functions as a Tumor Promoter via Recruiting Hepatoma-Derived Growth Factor/c-Jun Signal in Hepatocellular Carcinoma. | ||
Med Oncol The expression of hepatoma-derived growth factor in primary central nervous system lymphoma and its correlation with angiogenesis, proliferation and clinical outcome. | ||
J Biomed Res HDGF derived from Müller cells enhances the activation of microglia in diabetic retinopathy. | ||
Neural Plast Impact of Hepatoma-Derived Growth Factor Blockade on Resiniferatoxin-Induced Neuropathy. |
