HDGF Monoclonal antibody, PBS Only

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  WB analysis of SMMC-7721 using 60064-1-Ig (same clone as 60064-1-PBS)

  SMMC-7721 cells were subjected to SDS PAGE followed by western blot with 60064-1-Ig (HDGF Antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60064-1-PBS in a different storage buffer formulation.

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  WB analysis of rat liver using 60064-1-Ig (same clone as 60064-1-PBS)

  rat liver tissue were subjected to SDS PAGE followed by western blot with 60064-1-Ig (HDGF Antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60064-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 60064-1-Ig (same clone as 60064-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 60064-1-Ig (HDGF Antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60064-1-PBS in a different storage buffer formulation.

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  WB analysis of A549 using 60064-1-Ig (same clone as 60064-1-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 60064-1-Ig (HDGF Antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60064-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 60064-1-Ig (same clone as 60064-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 60064-1-Ig (HDGF Antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60064-1-PBS in a different storage buffer formulation.

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  WB analysis of L02 using 60064-1-Ig (same clone as 60064-1-PBS)

  L02 cells were subjected to SDS PAGE followed by western blot with 60064-1-Ig (HDGF Antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60064-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 60064-1-Ig (same clone as 60064-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 60064-1-Ig (HDGF Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60064-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 60064-1-Ig (same clone as 60064-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 60064-1-Ig (HDGF Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60064-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 60064-1-Ig (same clone as 60064-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 60064-1-Ig (HDGF Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 60064-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 60064-1-Ig (same clone as 60064-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 60064-1-Ig (HDGF Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 60064-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 60064-1-Ig (same clone as 60064-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60064-1-Ig (HDGF Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60064-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 60064-1-Ig (same clone as 60064-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60064-1-Ig (HDGF Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60064-1-PBS in a different storage buffer formulation.

HDGF Monoclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 60064-1-PBS

Clone No.5A7G3

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

5A7G3, Hepatoma-derived growth factor, High mobility group protein 1-like 2, HMG-1L2

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Product Information

60064-1-PBS targets HDGF in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag1893

  Product name: Recombinant human HDGF protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-240 aa of BC018991

  Sequence: MSRSNRQKEYKCGDLVFAKMKGYPHWPARIDEMPEAAVKSTANKYQVFFFGTHETAFLGPKDLFPYEESKEKFGKPNKRKGFSEGLWEIENNPTVKASGYQSSQKKSCVEEPEPEPEAAEGDGDKKGNAEGSSDEEGKLVIDEPAKEKNEKGALKRRAGDLLEDSPKRPKEAENPEGEEKEAATLEVERPLPMEVEKNSTPSEPGSGRGPPQEEEEEEDEEEEATKEDAEAPGIRDHESL

• Full Name: hepatoma-derived growth factor (high-mobility group protein 1-like)
• Calculated Molecular Weight: 26 kDa
• Observed Molecular Weight: 40 kDa
• GenBank Accession Number: BC018991
• Gene Symbol: HDGF
• Gene ID (NCBI): 3068
• RRID: AB_2117098
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P51858
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

HDGF, also named as HMG1L2, belongs to the HDGF family. It is a heparin-binding protein, with mitogenic activity for fibroblasts. HDGF acts as a transcriptional repressor. It plays important roles in the development and progression of cancers. HDGF may have a synergistic effect with VEGF during cancer development and Progression. (PMID:20848225)