KD/KO Validated

HDGF Monoclonal antibody

HDGF Monoclonal Antibody for WB, ELISA

Cat No. 60064-2-Ig

Clone No.1A10D5

Review Product

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, ELISA

Synonyms

Hepatoma-derived growth factor, High mobility group protein 1-like 2, HMG-1L2

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

SKU:

-/ -

Freight/Packing: -

Quantity

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WB analysis of HeLa using 60064-2-Ig
WB result of HDGF antibody (60064-2-Ig; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HDGF transfected HeLa cells.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis using 60064-2-Ig
Various lysates were subjected to SDS PAGE followed by western blot with 60064-2-Ig (HDGF antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis using 60064-2-Ig
Various lysates were subjected to SDS PAGE followed by western blot with 60064-2-Ig (HDGF antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Overview

Tested Applications
Product Information
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in

HT-29 cells, HeLa cells, Caki-1 cells, SCaBER cells, MG-63 cells, MCF-10A cells, MCF-7 cells, MOLT-4 cells, Karpas-299 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

60064-2-Ig targets HDGF in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Host / Isotype	Mouse / IgG2a
Class	Monoclonal
Type	Antibody
Immunogen	CatNo: Ag1893 Product name: Recombinant human HDGF protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-240 aa of BC018991 Sequence: MSRSNRQKEYKCGDLVFAKMKGYPHWPARIDEMPEAAVKSTANKYQVFFFGTHETAFLGPKDLFPYEESKEKFGKPNKRKGFSEGLWEIENNPTVKASGYQSSQKKSCVEEPEPEPEAAEGDGDKKGNAEGSSDEEGKLVIDEPAKEKNEKGALKRRAGDLLEDSPKRPKEAENPEGEEKEAATLEVERPLPMEVEKNSTPSEPGSGRGPPQEEEEEEDEEEEATKEDAEAPGIRDHESL Predict reactive species
Full Name	hepatoma-derived growth factor (high-mobility group protein 1-like)
Calculated Molecular Weight	26 kDa
Observed Molecular Weight	40 kDa
GenBank Accession Number	BC018991
Gene Symbol	HDGF
Gene ID (NCBI)	3068
RRID	AB_3743210
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	P51858
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HDGF, also named as HMG1L2, belongs to the HDGF family. It is a heparin-binding protein, with mitogenic activity for fibroblasts. HDGF acts as a transcriptional repressor. It plays important roles in the development and progression of cancers. HDGF may have a synergistic effect with VEGF during cancer development and Progression. (PMID:20848225)