HDGF Monoclonal antibody, PBS Only

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• HDGF Monoclonal antibody, PBS Only (60064-2-PBS)

HDGF Monoclonal Antibody for WB, Indirect ELISA

Cat No. 60064-2-PBS

Clone No.1A10D5

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, Indirect ELISA

Synonyms

Hepatoma-derived growth factor, High mobility group protein 1-like 2, HMG-1L2

• 

  WB analysis of HeLa using 60064-2-Ig (same clone as 60064-2-PBS)

  WB result of HDGF antibody (60064-2-Ig; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HDGF transfected HeLa cells. This data was developed using the same antibody clone with 60064-2-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis using 60064-2-Ig (same clone as 60064-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 60064-2-Ig (HDGF antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60064-2-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis using 60064-2-Ig (same clone as 60064-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 60064-2-Ig (HDGF antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60064-2-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Product Information

60064-2-PBS targets HDGF in WB, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag1893

  Product name: Recombinant human HDGF protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-240 aa of BC018991

  Sequence: MSRSNRQKEYKCGDLVFAKMKGYPHWPARIDEMPEAAVKSTANKYQVFFFGTHETAFLGPKDLFPYEESKEKFGKPNKRKGFSEGLWEIENNPTVKASGYQSSQKKSCVEEPEPEPEAAEGDGDKKGNAEGSSDEEGKLVIDEPAKEKNEKGALKRRAGDLLEDSPKRPKEAENPEGEEKEAATLEVERPLPMEVEKNSTPSEPGSGRGPPQEEEEEEDEEEEATKEDAEAPGIRDHESL

• Full Name: hepatoma-derived growth factor (high-mobility group protein 1-like)
• Calculated Molecular Weight: 26 kDa
• Observed Molecular Weight: 40 kDa
• GenBank Accession Number: BC018991
• Gene Symbol: HDGF
• Gene ID (NCBI): 3068
• RRID: AB_3743210
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P51858
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

HDGF, also named as HMG1L2, belongs to the HDGF family. It is a heparin-binding protein, with mitogenic activity for fibroblasts. HDGF acts as a transcriptional repressor. It plays important roles in the development and progression of cancers. HDGF may have a synergistic effect with VEGF during cancer development and Progression. (PMID:20848225)