Tested Applications
| Positive WB detected in | HaCaT cells, JAR cells, NCCIT cells, PC-3 cells, LNCaP cells, HeLa cells, K-562 cells |
| Positive IHC detected in | human breast cancer tissue, human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Mouse monoclonal antibodies of IgM isotype can be detected with "anti-mouse IgG (H+L)" secondary antibodies.
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
60281-1-Ig targets SECTM1 in WB, IHC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgM |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag14645 Product name: Recombinant human SECTM1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 29-248 aa of BC017716 Sequence: QNEGWDSPICTEGVVSVSWGENTVMSCNISNAFSHVNIKLRAHGQESAIFNEVAPGYFSRDGWQLQVQGGVAQLVIKGARDSHAGLYMWHLVGHQRNNRQVTLEVSGAEPQSAPDTGFWPVPAVVTAVFILLVALVMFAWYRCRCSQQRREKKFFLLEPQMKFAALRAGAQQGLSRASAELWTPDSEPTPRPLALVFKPSPLGALELLSPQPLFPYAADP Predict reactive species |
| Full Name | secreted and transmembrane 1 |
| Calculated Molecular Weight | 248 aa, 27 kDa |
| Observed Molecular Weight | 27 kDa |
| GenBank Accession Number | BC017716 |
| Gene Symbol | SECTM1 |
| Gene ID (NCBI) | 6398 |
| RRID | AB_2881399 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q8WVN6 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
SECTM1, also named as protein K12, is originally identified by its location just upstream of the CD7 locus. It has been proposed as a ligand for CD7. SECTM1 is involved in thymocyte signaling. This antibody is specific to SECTM1.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for SECTM1 antibody 60281-1-Ig | Download protocol |
| WB protocol for SECTM1 antibody 60281-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The SECTM1 Monoclonal antibody (clone 1C11B4), catalog number 60281-1-Ig, has 1 citation. It was cited in iScience (Impact Factor 4.5, published January 2023) for a study investigating SECTM1 upregulation in immuno-hot tumors and its role in predicting immunotherapeutic efficacy across multiple cancers. The research falls within the fields of cancer immunology and immunotherapy. The antibody was used in Western blot and IHC applications on human samples.
| Species | Application | Title |
|---|---|---|
iScience SECTM1 is upregulated in immuno-hot tumors and predicts immunotherapeutic efficacy in multiple cancers |
