Tested Applications
| Positive WB detected in | human liver tissue, pig liver tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
60345-1-Ig targets NR1H2 in WB, IHC, IF, ChIP, ELISA applications and shows reactivity with human, pig samples.
| Tested Reactivity | human, pig |
| Cited Reactivity | human, mouse |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag5635 Product name: Recombinant human NR1H2 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-461 aa of BC047750 Sequence: MSSPTTSSLDTPLPGNGPPQPGAPSSSPTVKEEGPEPWPGGPDPDVPGTDEASSACSTDWVIPDPEEEPERKRKKGPAPKMLGHELCRVCGDKASGFHYNVLSCEGCKGFFRRSVVRGGARRYACRGGGTCQMDAFMRRKCQQCRLRKCKEAGMREQCVLSEEQIRKKKIRKQQQQESQSQSQSPVGPQGSSSSASGPGASPGGSEAGSQGSGEGEGVQLTAAQELMIQQLVAAQLQCNKRSFSDQPKVTPWPLGADPQSRDARQQRFAHFTELAIISVQEIVDFAKQVPGFLQLGREDQIALLKASTIEIMLLETARRYNHETECITFLKDFTYSKDDFHRAGLQVEFINPIFEFSRAMRRLGLDDAEYALLIAINIFSADRPNVQEPGRVEALQQPYVEALLSYTRIKRPQDQLRFPRMLMKLVSLRTLSSVHSEQVFALRLQDKKLPPLLSEIWDVHE Predict reactive species |
| Full Name | nuclear receptor subfamily 1, group H, member 2 |
| Calculated Molecular Weight | 51 kDa |
| Observed Molecular Weight | 51 kDa |
| GenBank Accession Number | BC047750 |
| Gene Symbol | NR1H2 |
| Gene ID (NCBI) | 7376 |
| RRID | AB_2881454 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P55055 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
NR1H2, also known as LXRB, is a liver X receptors, which form a subfamily of the nuclear receptor superfamily and are key regulators of macrophage function, controlling transcriptional programs involved in lipid homeostasis and inflammation. It preferentially bind to double-stranded oligonucleotide direct repeats, which have the consensus half-site sequence 5'-AGGTCA-3' and 4-nt spacing (DR-4). It involves in the regulation of cholesterol uptake through MYLIP-dependent ubiquitination of LDLR, VLDLR and LRP8.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for NR1H2 antibody 60345-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The NR1H2 (LXRβ) Monoclonal antibody (1E12B10), catalog 60345-1-Ig, has 23 citations. These appear in journals including Nature Communications, Molecular Cell, Acta Pharmacologica Sinica, Journal of Lipid Research, Journal of Neuroinflammation, British Journal of Pharmacology, Neurotherapeutics, Journal of Biological Chemistry, Frontiers in Oncology, and Biochemical and Biophysical Research Communications. Research fields span lipid/cholesterol metabolism, atherosclerosis, liver disease (NASH, acute liver failure), cancer (hepatocellular, colorectal, prostate, gastric), neuroinflammation, Alzheimer's, and immune regulation.
| Species | Application | Title |
|---|---|---|
Nat Commun Oxysterol undersulfation promotes osteoarthritis by fueling chondrocyte lipid accumulation. | ||
Nat Commun MARCH2 prevents doxorubicin-induced cardiomyopathy by stabilizing NR1H2 and promoting clearance of apoptotic cardiomyocytes. | ||
Mol Cell mTOR inhibition reprograms cellular lipid homeostasis by inducing alternative lipid uptake and promoting cholesterol transport.
| ||
J Neuroinflammation Lysosomal cholesteryl ester hydrolysis drives white matter repair by reprogramming microglia into a novel reparative state. | ||
Acta Pharmacol Sin Betaine-homocysteine methyltransferase protects against acetaminophen-induced acute liver failure via BACH1-SCD1-oleic acid axis | ||
Acta Pharmacol Sin Liver X receptor agonists exert antitumor effects against hepatocellular carcinoma via inducing REPS2 expression |
Reviews
What customers say
The single review for this product is a 5-star rating. The reviewer used the antibody at a 1:1000 dilution for Western Blot on mouse liver tissue. They reported that it works well in liver lysates from both wild-type and hLXR knockin mice, indicating reliable performance for this application.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Zehua (Verified Customer) (11-07-2019) | This antibody works well in liver lysates from wild-type and hLXR knockin mouse.
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