Tested Applications
| Positive WB detected in | HepG2 cells, pig liver tissue, human testis tissue, L02 cells, human placenta tissue |
| Positive IHC detected in | human lung cancer tissue, human oesophagus cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
60352-1-Ig targets Inhibin beta A-Specific in WB, IHC, IF, ELISA applications and shows reactivity with human, pig samples.
| Tested Reactivity | human, pig |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | inhibin, beta A |
| Calculated Molecular Weight | 47 kDa |
| Observed Molecular Weight | 47 kDa |
| GenBank Accession Number | NM_002192 |
| Gene Symbol | Inhibin beta A |
| Gene ID (NCBI) | 3624 |
| RRID | AB_2881461 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P08476 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Inhibin βA(INHBA) is a ligand in the transforming growth factor β (TGF-β) superfamily. Inhibin βA forms a disulfide-linked homo-dimer known as activin A, which has been implicated in multiple biologic processes, including neoplastic progression. The inhibin βA can also bind the βB and α isoforms to form activin AB and inhibin, respectively.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for Inhibin beta A-Specific antibody 60352-1-Ig | Download protocol |
| WB protocol for Inhibin beta A-Specific antibody 60352-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The Inhibin beta A-Specific Monoclonal antibody (catalog number 60352-1-Ig) has 4 total citations. These appear in Cell Systems, Genomics Proteomics Bioinformatics, BMC Cancer, and bioRxiv. The research fields span single-cell cell-cell communication analysis, spatially resolved cell atlasing of esophageal squamous cell carcinoma, and immunosuppressive tumor microenvironment studies in oral squamous cell carcinoma. Applications include Western blot and immunofluorescence in human samples.
| Species | Application | Title |
|---|---|---|
Cell Syst Robust identification of cell-cell communication heterogeneity in single cells. | ||
Genomics Proteomics Bioinformatics A Single-cell and Spatially Resolved Cell Atlas of Human Esophageal Squamous Cell Carcinoma. | ||
BMC Cancer INHBA+ macrophages and Pro-inflammatory CAFs are associated with distinctive immunosuppressive tumor microenvironment in submucous Fibrosis-Derived oral squamous cell carcinoma | ||
