Tested Applications
| Positive FC detected in | LOU rat splenocytes |
Recommended dilution
| Application | Dilution |
|---|---|
| Flow Cytometry (FC) | FC : 0.5 ug per 10^6 cells in 100 μl suspension |
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
65229-1-Ig targets CD11b/c in IF, FC applications and shows reactivity with rat samples.
| Tested Reactivity | rat |
| Cited Reactivity | rat |
| Host / Isotype | Mouse / IgG2a, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
Rat peritoneal macrophages Predict reactive species |
| Full Name | integrin alpha M |
| Calculated Molecular Weight | 127 kDa |
| GenBank Accession Number | NM_012711 |
| Gene Symbol | CD11b |
| Gene ID (NCBI) | 25021 |
| RRID | AB_3084908 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Affinity purification |
| UNIPROT ID | Q9JI30 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at 2-8°C. Stable for one year after shipment. |
Background Information
Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated α and β subunits (PMID: 9779984). CD11b (integrin alpha M or CR3A) and CD11c (integrin alpha X) are members of the integrin alpha chain family and can form α/β heterodimers with CD18 (integrin β2). CD11b/CD18 is a receptor for the complement protein fragment C3bi, and is also a receptor for fibrinogen, factor X, and ICAM1 (PMID: 2971974; 15485828). CD11c/CD18 acts as a receptor for fibrinogen and is important in monocyte adhesion and chemotaxis (PMID: 1671533). The OX-42 antibody recognizes a common epitope shared between CD11b and CD11c. It binds to C3bi receptor and is known to block C3bi binding (PMID: 3512425; 8449218).
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for CD11b/c antibody 65229-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
Anti-Rat CD11b/c (OX42), catalog number 65229-1-Ig, has 2 published citations. These appear in Phytomedicine (IF 11.3) and Mater Today Bio (IF 11). The research fields covered are osteoarthritis and synovial inflammation (using IF application) and bone regeneration with immune balance and vascularization (using FC application), both in rat models.
| Species | Application | Title |
|---|---|---|
Phytomedicine Sclareol alleviates synovial inflammation in knee osteoarthritis by regulating sphingolipid metabolism along the gut-bone axis. | ||
Mater Today Bio 3D-printed nHA/PA66 porous scaffold: Regulating immune balance and vascularization synergistically promotes bone regeneration. |
