APC Anti-Rat CD11b/c (OX42)

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  FC experiment of rat bone marrow cells using APC-65229

  1X10^6 rat bone marrow cells were surface stained with 0.1 ug APC Anti-Rat CD11b/c (APC-65229, Clone: OX42) or 0.1 ug APC Mouse IgG2a Isotype Control (C1.18.4) (APC-65208, Clone: C1.18.4). Cells were not fixed.

CD11b/c Monoclonal Antibody for FC

Cat No. APC-65229

Clone No.OX42

Host / Isotype

Mouse / IgG2a, kappa

Reactivity

rat

Applications

FC

Synonyms

CD11b, integrin alpha M, Itgam, OX42

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Tested Applications

• Positive FC detected in: rat bone marrow cells

Recommended dilution

• Flow Cytometry (FC): FC : 0.1 ug per 10^6 cells in 100 μl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

APC-65229 targets CD11b/c in FC applications and shows reactivity with rat samples.

• Tested Reactivity: rat
• Host / Isotype: Mouse / IgG2a, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  Rat peritoneal macrophages

• Full Name: integrin alpha M
• Calculated Molecular Weight: 127 kDa
• GenBank Accession Number: NM_012711
• Gene Symbol: CD11b
• Gene ID (NCBI): 25021
• RRID: AB_3084945
• Conjugate: APC Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 650 nm / 660 nm
• Form: Liquid
• Purification Method: N/A
• UNIPROT ID: Q9JI30
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated α and β subunits (PMID: 9779984). CD11b (integrin alpha M or CR3A) and CD11c (integrin alpha X) are members of the integrin alpha chain family and can form α/β heterodimers with CD18 (integrin β2). CD11b/CD18 is a receptor for the complement protein fragment C3bi, and is also a receptor for fibrinogen, factor X, and ICAM1 (PMID: 2971974; 15485828). CD11c/CD18 acts as a receptor for fibrinogen and is important in monocyte adhesion and chemotaxis (PMID: 1671533). The OX-42 antibody recognizes a common epitope shared between CD11b and CD11c. It binds to C3bi receptor and is known to block C3bi binding (PMID: 3512425; 8449218).