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  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human IL-1 beta (2A7C6) (65276-1-Ig)

    FC experiment of human PBMCs using 65276-1-Ig (same clone as 65276-1-PBS)

    1X10^6 LPS and Brefeldin A treated human PBMCs were surface stained with Anti-Human HLA-DR then intracellularly stained with 0.2 ug CoraLite® Plus 488 Anti-Human IL-1β (CL488-65276, Clone:2A7C6) or Mouse IgG1 Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). Monocytes were gated. This data was developed using the CoraLite ® Plus 488 conjugated antibody of 65276-1-Ig. This data was developed using the same antibody clone with 65276-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human IL-1 beta (2A7C6) (65276-1-Ig)

    FC experiment of human PBMCs using 65276-1-Ig (same clone as 65276-1-PBS)

    1X10^6 LPS and Brefeldin A treated human PBMCs were intracellularly stained with 0.2 ug CoraLite® Plus 488 Anti-Human IL-1β (CL488-65276, Clone:2A7C6) (red) or Mouse IgG1 Isotype Control (blue), or unactivated and Brefeldin A treated human PBMCs were intracellularly stained with 0.2 ug CoraLite® Plus 488 Anti-Human IL-1β (CL488-65276, Clone:2A7C6) (black, dashed line). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). Monocytes were gated. This data was developed using the CoraLite ® Plus 488 conjugated antibody of 65276-1-Ig. This data was developed using the same antibody clone with 65276-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 65276-1-Ig

    Affinity and Kinetic Characterization of 65276-1-Ig

    Biolayer interferometry (BLl) kinetic assays of 65276-1-Ig against IL-1 beta were performed. The affinity constant is 102 nM.

Anti-Human IL-1 beta (2A7C6), PBS Only

IL-1 beta Monoclonal Antibody for FC (Intra), Indirect ELISA
Cat No. 65276-1-PBS
Clone No.2A7C6

Review Product

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

FC (Intra), Indirect ELISA

Synonyms

2A7C6, Catabolin, IL 1, IL 1 beta, IL1 beta

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

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Product Information

65276-1-PBS targets IL-1 beta in FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen IL-1 beta fusion protein HZ-1164 Predict reactive species
Full Name interleukin 1, beta
Calculated Molecular Weight 269 aa, 31 kDa
GenBank Accession NumberBC008678
Gene Symbol IL1B
Gene ID (NCBI) 3553
ENSEMBL Gene IDENSG00000125538
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS only, pH 7.3.
Storage ConditionsStore at -80°C.

Background Information

Interleukin-1 is a pro-inflammatory cytokine with multiple biological effects. The IL-1 gene family encodes three proteins: IL-1α, IL-1β and their naturally occurring inhibitor Il-1RN. Interleukin 1β (IL-1β), mainly produced by blood monocytes and tissue macrophages, has been implicated in mediating both acute and chronic inflammation. IL-1β is known to be involved in a variety of cellular activities, including cell proliferation, differentiation and apoptosis. IL-1β is emerging as a key mediator of carcinogenesis that characterizes host-environment interactions. (PMID: 8630372; 12401481; 23704929; 24618930)