FC experiment of Human Bone marrow mesenchymal stromal cells using 65585-1-MR
1x10^6 human bone marrow mesenchymal stromal cells were surface stained with 0.25 ug Anti-Human STRO-1 (STRO-1) Mouse IgG2a RecAb (65585-1-MR, Clone: STRO-1) or 0.25 ug Mouse IgG2a Isotype Control (C1.18.4) (65571-1-Ig, Clone: MOPC-173), and PE-conjugated Goat Anti-Mouse IgG. Cells were co-stained with 0.25 ug CoraLite® Plus 405 Anti-Human CD90 Rabbit Recombinant Antibody (CL405-98126, Clone: 241290B2). Cells were not fixed.
1x10^6 human bone marrow mesenchymal stromal cells were surface stained with 0.25 ug Anti-Human STRO-1 (STRO-1) Mouse IgG2a RecAb (65585-1-MR, Clone: STRO-1) or 0.25 ug Mouse IgG2a Isotype Control (C1.18.4) (65571-1-Ig, Clone: MOPC-173), and PE-conjugated Goat Anti-Mouse IgG. Cells were co-stained with 0.25 ug CoraLite® Plus 405 Anti-Human CD90 Rabbit Recombinant Antibody (CL405-98126, Clone: 241290B2). Cells were not fixed.
FC experiment of human blood using 65585-1-MR
1x10^6 human blood cells were surface unstained or stained with 0.5 ug Anti-Human STRO-1 Mouse IgG2a Recombinant Antibody (65585-1-MR, Clone: STRO-1) and CoraLite®488-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L). Cells were not fixed. CD45 dim cells were gated.
1x10^6 human blood cells were surface unstained or stained with 0.5 ug Anti-Human STRO-1 Mouse IgG2a Recombinant Antibody (65585-1-MR, Clone: STRO-1) and CoraLite®488-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L). Cells were not fixed. CD45 dim cells were gated.
FC experiment of human blood using 65585-1-MR
1x10^6 human blood cells were unstained (blue) or surface stained with 0.5 ug Anti-Human STRO-1 Mouse IgG2a Recombinant Antibody (65585-1-MR, Clone: STRO-1) and CoraLite®488-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L) (red). Cells were not fixed. CD45 dim cells were gated.
1x10^6 human blood cells were unstained (blue) or surface stained with 0.5 ug Anti-Human STRO-1 Mouse IgG2a Recombinant Antibody (65585-1-MR, Clone: STRO-1) and CoraLite®488-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L) (red). Cells were not fixed. CD45 dim cells were gated.
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human bone marrow mesenchymal stromal cells, human blood cells
Recommended dilution
Application
Dilution
Flow Cytometry (FC)
FC : 0.25 ug per 10^6 cells in a 100 µl suspension
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
Product Information
65585-1-MR targets STRO-1 in FC applications and shows reactivity with human samples.
FC experiment of Human Bone marrow mesenchymal stromal cells using 65585-1-MR
1x10^6 human bone marrow mesenchymal stromal cells were surface stained with 0.25 ug Anti-Human STRO-1 (STRO-1) Mouse IgG2a RecAb (65585-1-MR, Clone: STRO-1) or 0.25 ug Mouse IgG2a Isotype Control (C1.18.4) (65571-1-Ig, Clone: MOPC-173), and PE-conjugated Goat Anti-Mouse IgG. Cells were co-stained with 0.25 ug CoraLite® Plus 405 Anti-Human CD90 Rabbit Recombinant Antibody (CL405-98126, Clone: 241290B2). Cells were not fixed.
FC experiment of human blood using 65585-1-MR
1x10^6 human blood cells were surface unstained or stained with 0.5 ug Anti-Human STRO-1 Mouse IgG2a Recombinant Antibody (65585-1-MR, Clone: STRO-1) and CoraLite®488-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L). Cells were not fixed. CD45 dim cells were gated.
FC experiment of human blood using 65585-1-MR
1x10^6 human blood cells were unstained (blue) or surface stained with 0.5 ug Anti-Human STRO-1 Mouse IgG2a Recombinant Antibody (65585-1-MR, Clone: STRO-1) and CoraLite®488-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L) (red). Cells were not fixed. CD45 dim cells were gated.
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
STRO-1 Recombinant antibody
Catalog Number
65585-1-MR
Citations
-
Dilutions
Applications
FC
Reactivity
human
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
