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NOB1 Monoclonal antibody, PBS Only (Capture) (66048-1-PBS)

NOB1 Monoclonal antibody, PBS Only (Capture)

Name: NOB1 Monoclonal antibody, PBS Only (Capture), 100ug
Brand: Proteintech
SKU: 66048-1-PBS-100UG

NOB1 Monoclonal Antibody for WB, IHC, Cytometric bead array, Indirect ELISA

Cat No. 66048-1-PBS

Clone No. 8C11A12

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Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Synonyms

ART 4, ART4, EC:3.1.-.-, MST158, MSTP158

Formulation: PBS Only

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Size/Concentration:

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Filter: AllWBIHCCytometric bead array sample testCytometric bead array

WB analysis of Jurkat using 66048-1-Ig (same clone as 66048-1-PBS)
Jurkat cells were subjected to SDS PAGE followed by western blot with 66048-1-Ig (NOB1 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66048-1-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of human placenta using 66048-1-Ig (same clone as 66048-1-PBS)
human placenta tissue were subjected to SDS PAGE followed by western blot with 66048-1-Ig (NOB1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66048-1-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human breast cancer using 66048-1-Ig (same clone as 66048-1-PBS)
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66048-1-Ig (NOB1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66048-1-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human breast cancer using 66048-1-Ig (same clone as 66048-1-PBS)
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66048-1-Ig (NOB1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66048-1-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
Cytometric bead array sample test of MP51874-1
Cytometric bead array sample test of MP51874-1, NOB1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66048-1-PBS. Detection antibody: 66048-3-PBS.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
Cytometric bead array standard curve of MP51874-1
Cytometric bead array standard curve of MP51874-1, NOB1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66048-1-PBS. Detection antibody: 66048-3-PBS. Standard:Ag17907. Range: 0.781-100 ng/mL.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Overview

Tested Applications
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Product Information

66048-1-PBS targets NOB1 as part of a matched antibody pair:

MP51874-1: 66048-1-PBS capture and 66048-3-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity	human
Host / Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Immunogen	CatNo: Ag17907 Product name: Recombinant human NOB1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 36-293 aa of BC000050 Sequence: SSFMFWRNPLPNIDHELQELLIDRGEDVPSEEEEEEENGFEDRKDDSDDDGGGWITPSNIKQIQQELEQCDVPEDVRVGCLTTDFAMQNVLLQMGLHVLAVNGMLIREARSYILRCHGCFKTTSDMSRVFCSHCGNKTLKKVSVTVSDDGTLHMHFSRNPKVLNPRGLRYSLPTPKGGKYAINPHLTEDQRFPQLRLSQKARQKTNVFAPDYIAGVSPFVENDISSRSATLQVRDSTLGAGRRRLNPNASRKKFVKKR Predict reactive species
Full Name	NIN1/RPN12 binding protein 1 homolog (S. cerevisiae)
Calculated Molecular Weight	47 kDa
Observed Molecular Weight	47 kDa
GenBank Accession Number	BC000050
Gene Symbol	NOB1
Gene ID (NCBI)	28987
RRID	AB_11042447
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein G purification
UNIPROT ID	Q9ULX3
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

NOB1 was first identified in yeast as an essential gene encoding the Nin one binding protein, which is involved in pre-rRNA processing. In a late cytoplasmic processing step, Nob1 cleaves a 20S rRNA intermediate at cleavage site D to produce the mature 18S rRNA. In addition, NOB1 is a crucial molecule in the maturation of the 20S proteasome and protein degradation. It serves as a chaperone to join the 20S proteasome with the 19S regulatory particle in the nucleus and facilitates the maturation of the 20S proteasome. Recently NOB1 has been reported to be overexpressed in several types of cancer, suggesting its involvement in the tumorigenesis.