Tested Applications
| Positive WB detected in | PC-3 cells, HEK-293 cells, LNCaP cells, HeLa cells, Jurkat cells, K-562 cells, HSC-T6 cells, PC-12 cells, NIH/3T3 cells |
| Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:20000-1:100000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
66055-1-Ig targets PA2G4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag7850 Product name: Recombinant human PA2G4 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-394 aa of BC001951 Sequence: MSGEDEQQEQTIAEDLVVTKYKMGGDIANRVLRSLVEASSSGVSVLSLCEKGDAMIMEETGKIFKKEKEMKKGIAFPTSISVNNCVCHFSPLKSDQDYILKEGDLVKIDLGVHVDGFIANVAHTFVVDVAQGTQVTGRKADVIKAAHLCAEAALRLVKPGNQNTQVTEAWNKVAHSFNCTPIEGMLSHQLKQHVIDGEKTIIQNPTDQQKKDHEKAEFEVHEVYAVDVLVSSGEGKAKDAGQRTTIYKRDPSKQYGLKMKTSRAFFSEVERRFDAMPFTLRAFEDEKKARMGVVECAKHELLQPFNVLYEKEGEFVAQFKFTVLLMPNGPMRITSGPFEPDLYKSEMEVQDAELKALLQSSASRKTQKKKKKKASKTAENATSGETLEENEAGD Predict reactive species |
| Full Name | proliferation-associated 2G4, 38kDa |
| Calculated Molecular Weight | 44 kDa |
| Observed Molecular Weight | 38-42 kDa, 48 kDa |
| GenBank Accession Number | BC001951 |
| Gene Symbol | PA2G4 |
| Gene ID (NCBI) | 5036 |
| RRID | AB_11042597 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q9UQ80 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
PA2G4 (EBP1), first identified as an ErbB3 binding protein, is a 38 kDa protein that is widely expressed in cultured cells and tissues. The presence of several functional domains such as a nuclear localization sequence (NLS), LxxLL and LxCxE suggests the involvement of Ebp1 in cell signaling pathways and gene transcription regulation. This antibody is a mouse mono-clonal antibody raised against full length PA2G4 of human origin.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for PA2G4 antibody 66055-1-Ig | Download protocol |
| IHC protocol for PA2G4 antibody 66055-1-Ig | Download protocol |
| WB protocol for PA2G4 antibody 66055-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The PA2G4 Monoclonal antibody (Cat# 66055-1-Ig) has 4 citations. These appear in Cell Stem Cell, Nature Communications, Nucleic Acids Research, and Nature Cardiovascular Research. The associated research fields include hematopoietic stem cell regeneration, ribosome assembly and protein synthesis, the druggable PA2G4-MYC axis in acute myeloid leukemia, viral IRES-driven translation initiation, and PA2G4-mediated heart regeneration in mice. All citations used the antibody for Western blot (WB) applications.
| Species | Application | Title |
|---|---|---|
Cell Stem Cell HectD1 controls hematopoietic stem cell regeneration by coordinating ribosome assembly and protein synthesis. | ||
Nat Commun Orthogonal proteogenomic analysis identifies the druggable PA2G4-MYC axis in 3q26 AML | ||
Nucleic Acids Res The p48 isoform of the PA2G4/EBP1/ITAF45 oncoprotein is required for the encephalomyocarditis virus IRES-driven translation initiation.
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Nat Cardiovasc Res The highly conserved PIWI-interacting RNA CRAPIR antagonizes PA2G4-mediated NF110-NF45 disassembly to promote heart regeneration in mice
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