PA2G4 Monoclonal antibody, PBS Only (Detector)

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  WB analysis using 66055-1-Ig (same clone as 66055-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66055-1-Ig (PA2G4 antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66055-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 66055-1-Ig (same clone as 66055-1-PBS)

  WB result of PA2G4 antibody (66055-1-Ig; 1:100000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PA2G4 transfected HEK-293 cells. This data was developed using the same antibody clone with 66055-1-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP50029-1

  Sandwich ELISA standard curve of MP50029-1, PA2G4 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66055-2-PBS. Detection antibody: HRP-conjugated 66055-1-PBS. Standard: Ag7850. Range: 1.25-20 ng/mL.

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  IHC staining of human breast cancer using 66055-1-Ig (same clone as 66055-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66055-1-Ig (PA2G4 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66055-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66055-1-Ig (same clone as 66055-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66055-1-Ig (PA2G4 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66055-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 66055-1-Ig (same clone as 66055-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using PA2G4 antibody (66055-1-Ig, Clone: 3G10D9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66055-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

PA2G4 Monoclonal Antibody for WB, IHC, IF/ICC, Sandwich ELISA, Indirect ELISA

Cat No. 66055-1-PBS

Clone No.3G10D9

Host / Isotype

Mouse / IgG2a

Reactivity

human, rat, mouse

Applications

WB, IHC, IF/ICC, Sandwich ELISA, Indirect ELISA

Synonyms

EBP1, ErbB3 binding protein 1, HG4 1, p38 2G4, PA2G4

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Product Information

66055-1-PBS targets PA2G4 as part of a matched antibody pair:

MP50029-1: 66055-2-PBS capture and 66055-1-PBS detection (validated in Sandwich ELISA)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human, rat, mouse
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag7850

  Product name: Recombinant human PA2G4 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-394 aa of BC001951

  Sequence: MSGEDEQQEQTIAEDLVVTKYKMGGDIANRVLRSLVEASSSGVSVLSLCEKGDAMIMEETGKIFKKEKEMKKGIAFPTSISVNNCVCHFSPLKSDQDYILKEGDLVKIDLGVHVDGFIANVAHTFVVDVAQGTQVTGRKADVIKAAHLCAEAALRLVKPGNQNTQVTEAWNKVAHSFNCTPIEGMLSHQLKQHVIDGEKTIIQNPTDQQKKDHEKAEFEVHEVYAVDVLVSSGEGKAKDAGQRTTIYKRDPSKQYGLKMKTSRAFFSEVERRFDAMPFTLRAFEDEKKARMGVVECAKHELLQPFNVLYEKEGEFVAQFKFTVLLMPNGPMRITSGPFEPDLYKSEMEVQDAELKALLQSSASRKTQKKKKKKASKTAENATSGETLEENEAGD

• Full Name: proliferation-associated 2G4, 38kDa
• Calculated Molecular Weight: 44 kDa
• Observed Molecular Weight: 38-42 kDa, 48 kDa
• GenBank Accession Number: BC001951
• Gene Symbol: PA2G4
• Gene ID (NCBI): 5036
• RRID: AB_11042597
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9UQ80
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

PA2G4 (EBP1), first identified as an ErbB3 binding protein, is a 38 kDa protein that is widely expressed in cultured cells and tissues. The presence of several functional domains such as a nuclear localization sequence (NLS), LxxLL and LxCxE suggests the involvement of Ebp1 in cell signaling pathways and gene transcription regulation. This antibody is a mouse mono-clonal antibody raised against full length PA2G4 of human origin.