Tested Applications
| Positive WB detected in | PC-3 cells, A431 cells, A549 cells, HepG2 cells, mouse brain tissue |
| Positive IHC detected in | human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:2000-1:8000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
66070-1-Ig targets Integrin Alpha 3 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, pig, mouse samples.
| Tested Reactivity | human, pig, mouse |
| Cited Reactivity | human, mouse, rat, pig, rabbit |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag17293 Product name: Recombinant human Integrin alpha-3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 52-376 aa of BC150190 Sequence: FGYSVALHRQTERQQRYLLLAGAPRELAVPDGYTNRTGAVYLCPLTAHKDDCERMNITVKNDPGHHIIEDMWLGVTVASQGPAGRVLVCAHRYTQVLWSGSEDQRRMVGKCYVRGNDLELDSSDDWQTYHNEMCNSNTDYLETGMCQLGTSGGFTQNTVYFGAPGAYNWKGNSYMIQRKEWDLSEYSYKDPEDQGNLYIGYTMQVGSFILHPKNITIVTGAPRHRHMGAVFLLSQEAGGDLRRRQVLEGSQVGAYFGSAIALADLNNDGWQDLLVGAPYYFERKEEVGGAIYVFMNQAGTSFPAHPSLLLHGPSGSAFGLSVASI Predict reactive species |
| Full Name | integrin, alpha 3 (antigen CD49C, alpha 3 subunit of VLA-3 receptor) |
| Calculated Molecular Weight | 1066 aa, 119 kDa |
| Observed Molecular Weight | 120 kDa |
| GenBank Accession Number | BC150190 |
| Gene Symbol | Integrin alpha 3 |
| Gene ID (NCBI) | 3675 |
| RRID | AB_11042765 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P26006 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
ITGA3, also named as MSK18, CD49c, GAPB3 and FRP-2, belongs to the integrin alpha chain family. It is a receptor for fibronectin, laminin, collagen, epiligrin, thrombospondin and CSPG4. ITGA3 may mediate with LGALS3 the stimulation by CSPG4 of endothelial cells migration. ITGA3 is highly concentrated in epithelial cells where it strongly adheres to Laminin-5 and Laminin-5 induced rapid adhesion can be blocked by antibodies against the alpha-3 integrin subunit. It can be cleaved into heavy chain(840aa) and light chain(176aa). This antibody can recognize the heavy chain.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for Integrin Alpha 3 antibody 66070-1-Ig | Download protocol |
| WB protocol for Integrin Alpha 3 antibody 66070-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The Integrin Alpha 3 Monoclonal antibody (catalog #66070-1-Ig) has 37 citations. These appear in journals including Nature, Cell, Nature Cell Biology, Nature Communications, Advanced Science, PNAS, Cell Reports, iScience, FASEB Journal, Development, and others. Research fields span cancer biology (hepatocellular, pancreatic, gastric, cervical, glioblastoma, and other carcinomas), wound healing, fibrosis, kidney disease, vascular biology, reproductive biology, and integrin signaling pathways. Applications include WB, IHC, IF, IP, and flow cytometry.
| Species | Application | Title |
|---|---|---|
Nat Cell Biol Growth factor-triggered de-sialylation controls glycolipid-lectin-driven endocytosis | ||
Nat Commun Galectin-3-integrin α5β1 phase separation disrupted by advanced glycation end-products impairs diabetic wound healing in rodents. | ||
Adv Sci (Weinh) Biodegradable Cardiac Occluder with Surface Modification by Gelatin-Peptide Conjugate to Promote Endogenous Tissue Regeneration | ||
Carbohydr Polym High molecular weight laminarin/AgNPs-impregnated PVA based in situ hydrogels accelerated diabetic wound healing. |
Reviews
What customers say
Customers report positive experiences with this antibody. One user rated it 5 stars for Western blot, noting it produced a clean band at the correct molecular weight using a 1/1000 overnight dilution at 4 °C on MDA-MB-231 cells. Two additional users rated it 4 stars each after testing it with EDTA plasma on an antibody microarray. Overall, the reviews reflect reliable performance across applications, with particular praise for specific, clean detection in Western blot.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Ewan (Verified Customer) (07-11-2024) | Works well by western clean band and correct size
|
Carly (Verified Customer) (11-17-2020) | Tested using EDTA plasma on an antibody microarray
|
Carly (Verified Customer) (11-17-2020) | Tested using EDTA plasma on an antibody microarray
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