Tested Applications
| Positive WB detected in | HeLa cells, HEK293 cells |
| Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
66100-1-Ig targets PDCD4 in WB, IHC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag19239 Product name: Recombinant human PDCD4 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 169-469 aa of BC026104 Sequence: TPIIQEYFEHGDTNEVAEMLRDLNLGEMKSGVPVLAVSLALEGKASHREMTSKLLSDLCGTVMSTTDVEKSFDKLLKDLPELALDTPRAPQLVGQFIARAVGDGILCNTYIDSYKGTVDCVQARAALDKATVLLSMSKGGKRKDSVWGSGGGQQSVNHLVKEIDMLLKEYLLSGDISEAEHCLKELEVPHFHHELVYEAIIMVLESTGESTFKMILDLLKSLWKSSTITVDQMKRGYERIYNEIPDINLDVPHSYSVLERFVEECFQAGIISKQLRDLCPSRGRKRFVSEGDGGRLKPESY Predict reactive species |
| Full Name | programmed cell death 4 (neoplastic transformation inhibitor) |
| Calculated Molecular Weight | 469 aa, 52 kDa |
| Observed Molecular Weight | 56 kDa |
| GenBank Accession Number | BC026104 |
| Gene Symbol | PDCD4 |
| Gene ID (NCBI) | 27250 |
| RRID | AB_2881499 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q53EL6 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Programmed cell death 4 (Pdcd4) is a novel tumor suppressor that inhibits translation, progression and invasion. It was first identified as being differnetially upregulated during apoptosis. Pdcd4 interferes with the activity of the eukaryotic initiation factor (eIF) 4A by displacing the scaffold protein eIF4G from its binding to the RNA helicase eIF4A.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for PDCD4 antibody 66100-1-Ig | Download protocol |
| WB protocol for PDCD4 antibody 66100-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The PDCD4 Monoclonal antibody (1F1A11), catalog number 66100-1-Ig, has 4 total citations. These appear in J Med Chem, iScience, J Tradit Chin Med, and Clin Res Hepatol Gastroenterol. The associated research fields span hepatocellular carcinoma (microRNA biogenesis and lncRNA/miR-93-5p/PDCD4 pathway), leukemogenesis (NPM1-mediated PDCD4 ubiquitination), and tongue squamous cell carcinoma (microRNA-21 down-regulation). All citations used the antibody for Western blot in human samples.
| Species | Application | Title |
|---|---|---|
J Med Chem Discovery of a Novel Small-Molecule Inhibitor Disrupting TRBP-Dicer Interaction against Hepatocellular Carcinoma via the Modulation of microRNA Biogenesis. | ||
iScience Mutant NPM1 modulates PDCD4 ubiquitination degradation and facilitates leukemogenesis.
| ||
Clin Res Hepatol Gastroenterol Long non-coding RNA LINC00472 suppresses hepatocellular carcinoma cell proliferation, migration and invasion through miR-93-5p/PDCD4 pathway. | ||
J Tradit Chin Med Qilan preparation inhibits proliferation and induces apoptosis by down-regulating microRNA-21 in human Tca8113 tongue squamous cell carcinoma cells |
Reviews
What customers say
The single review for this product is a 5-star rating for Western Blot. The reviewer found the antibody suitable for WB, noting that the suggested concentration (1:4000) and incubation times worked well on primary mouse T cells. Overall, the feedback is positive, confirming reliable performance with the manufacturer's recommended protocol.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Mirna (Verified Customer) (06-26-2025) | Antibody is suitable for western blot. The suggested concentration and the incubation times online worked well.
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