Tested Applications
| Positive WB detected in | Raji cells |
| Positive IHC detected in | human lung cancer tissue, human small intestine tissue, human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | human breast cancer tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:600 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
66190-1-Ig targets IL-22RA2 in WB, IHC, IF-P, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, pig |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag18682 Product name: Recombinant human IL-22RA2 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-232 aa of BC125167 Sequence: MMPKHCFLGFLISFFLTGVAGTQSTHESLKPQRVQFQSRNFHNILQWQPGRALTGNSSVYFVQYKIYGQRQWKNKEDCWGTQELSCDLTSETSDIQEPYYGRVRAASAGSYSEWSMTPRFTPWWETKIDPPVMNITQVNGSLLVILHAPNLPYRYQKEKNVSIEDYYELLYRVFIINNSLEKEQKVYEGAHRAVEIEALTPHSSYCVVAEIYQPMLDRRSQRSEERCVEIP Predict reactive species |
| Full Name | interleukin 22 receptor, alpha 2 |
| Calculated Molecular Weight | 263 aa, 31 kDa |
| Observed Molecular Weight | 35 kDa |
| GenBank Accession Number | BC125167 |
| Gene Symbol | IL-22RA2 |
| Gene ID (NCBI) | 116379 |
| RRID | AB_2881585 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q969J5 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Interleukin-22 receptor subunit alpha-2 (IL22RA2) is a soluble member of the type II cytokine receptor family. Through binding to IL22, IL22RA2 prevents the interaction of IL22 with the functional IL22-R complex and blocks the activity of IL22 (in vitro). IL22RA2 may play an important role as an IL22 antagonist in the regulation of inflammatory responses. Alternative splicing results in multiple transcript variants encoding distinct isoforms.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for IL-22RA2 antibody 66190-1-Ig | Download protocol |
| IHC protocol for IL-22RA2 antibody 66190-1-Ig | Download protocol |
| WB protocol for IL-22RA2 antibody 66190-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The antibody 66190-1-Ig has 4 total citations published in Acta Pharmacologica Sinica, Frontiers in Immunology, Cells, and Frontiers in Cellular and Infection Microbiology. Research fields span neuropharmacology (FSHR ablation and depression-like behaviors), immunology (unfolded protein response activation by IL-22BP), genetics/neurology (IL-22RA2 variant and multiple sclerosis risk), and infectious disease (Salmonella-induced inflammation in pig models via the IL-22BP/IL-22/STAT3 pathway).
| Species | Application | Title |
|---|---|---|
Front Immunol Long Interleukin-22 Binding Protein Isoform-1 Is an Intracellular Activator of the Unfolded Protein Response. | ||
Cells The Rare IL22RA2 Signal Peptide Coding Variant rs28385692 Decreases Secretion of IL-22BP Isoform-1, -2 and -3 and Is Associated with Risk for Multiple Sclerosis. | ||
Front Cell Infect Microbiol Oral Administration of Lactobacillus rhamnosus GG Ameliorates Salmonella Infantis-Induced Inflammation in a Pig Model via Activation of the IL-22BP/IL-22/STAT3 Pathway. |
