Tested Applications
| Positive WB detected in | HeLa cells, HepG2 cells, MCF-7 cells, HEK-293 cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells |
| Positive IHC detected in | human ovary tumor tissue, human cervical cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:2000-1:6000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
66192-1-Ig targets ERK1/2 in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag20350 Product name: Recombinant human ERK1/2 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-379 aa of BC013992 Sequence: MAAAAAQGGGGGEPRRTEGVGPGVPGEVEMVKGQPFDVGPRYTQLQYIGEGAYGMVSSAYDHVRKTRVAIKKISPFEHQTYCQRTLREIQILLRFRHENVIGIRDILRASTLEAMRDVYIVQDLMETDLYKLLKSQQLSNDHICYFLYQILRGLKYIHSANVLHRDLKPSNLLINTTCDLKICDFGLARIADPEHDHTGFLTEYVATRWYRAPEIMLNSKGYTKSIDIWSVGCILAEMLSNRPIFPGKHYLDQLNHILGILGSPSQEDLNCIINMKARNYLQSLPSKTKVAWAKLFPKSDSKALDLLDRMLTFNPNKRITVEEALAHPYLEQYYDPTDEPVAEEPFTFAMELDDLPKERLKELIFQETARFQPGVLEAP Predict reactive species |
| Full Name | mitogen-activated protein kinase 3 |
| Calculated Molecular Weight | 43 kDa |
| Observed Molecular Weight | 38-44 kDa |
| GenBank Accession Number | BC013992 |
| Gene Symbol | ERK1 |
| Gene ID (NCBI) | 5595 |
| RRID | AB_2811205 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P27361 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
ERK1 and ERK2 belongs to the protein kinase superfamily. It is involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK-1. ERK1/2 catalized the reaction: ATP + a protein = ADP + a phosphoprotein. It is activated by tyrosine phosphorylation in response to INS and NGF. This antibody can recognize both ERK1 and ERK2 with the molecular mass of 38-44 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for ERK1/2 antibody 66192-1-Ig | Download protocol |
| IHC protocol for ERK1/2 antibody 66192-1-Ig | Download protocol |
| WB protocol for ERK1/2 antibody 66192-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The ERK1/2 Monoclonal antibody (catalog number 66192-1-Ig) has 101 citations. These appear across journals including Advanced Science, Carbohydrate Polymers, Cell Reports, Oncogene, Nature Communications, Acta Pharmacologica Sinica, International Journal of Biological Sciences, Journal of Translational Medicine, Diabetes, Developmental Cell, and Scientific Reports, among others. Research fields span oncology (lung, liver, colorectal, breast, gastric cancers), neuroscience (Parkinson's, Alzheimer's, stroke), immunology, inflammation, metabolism, fibrosis, and MAPK/ERK signaling studies, primarily using Western blot applications.
| Species | Application | Title |
|---|---|---|
Adv Sci (Weinh) EGFR Targeted Liposomal PROTAC Assisted With Epigenetic Regulation as an Efficient Strategy for Osimertinib-Resistant Lung Cancer Therapy | ||
Adv Sci (Weinh) Ccl2-Induced Regulatory T Cells Balance Inflammation Through Macrophage Polarization During Liver Reconstitution | ||
Carbohydr Polym Yeast β-glucan-based nanoparticles loading methotrexate promotes osteogenesis of hDPSCs and periodontal bone regeneration under the inflammatory microenvironment | ||
Research (Wash D C) SH2B1 Tunes Hippocampal ERK Signaling to Influence Fluid Intelligence in Humans and Mice | ||
Cell Mol Biol Lett The promoting roles of GLP1R and GIPR in stemness maintenance and multiple lineage-specific differentiation of PDLSCs. | ||
Pharmacol Res Anti-thrombotic effects mediated by dihydromyricetin involve both platelet inhibition and endothelial protection. |
Reviews
What customers say
The single review for this ERK1/2 mouse monoclonal antibody (lot 10006691) reports a 5-star rating. The user applied it by Western Blot on extracts from cultured primary mouse hippocampal neurons using HRP detection at a 1:2000 dilution, and described the result as a clean signal. Overall, the feedback is positive, highlighting reliable performance and clear detection in this neuronal application.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Juliette (Verified Customer) (03-02-2026) | Clean signal for this ERK1/2 Mouse McAb used on extracts from cultured primary mouse hippocampal neurons, with an HRP revelation.
|
