Tested Applications
| Positive WB detected in | ACHN cells, human testis tissue, fetal human brain tissue, HepG2 cells, HuH-7 cells, pig liver tissue, rat brain tissue, rat liver tissue, mouse kidney tissue, rabbit kidney tissue |
| Positive IHC detected in | mouse testis tissue, human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
66193-1-Ig targets GSNOR,ADH5 in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.
| Tested Reactivity | human, mouse, rat, pig, rabbit |
| Cited Reactivity | mouse, rat, canine |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag9511 Product name: Recombinant human GSNOR,ADH5 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-374 aa of BC014665 Sequence: MANEVIKCKAAVAWEAGKPLSIEEIEVAPPKAHEVRIKIIATAVCHTDAYTLSGADPEGCFPVILGHEGAGIVESVGEGVTKLKAGDTVIPLYIPQCGECKFCLNPKTNLCQKIRVTQGKGLMPDGTSRFTCKGKTILHYMGTSTFSEYTVVADISVAKIDPLAPLDKVCLLGCGISTGYGAAVNTAKLEPGSVCAVFGLGGVGLAVIMGCKVAGASRIIGVDINKDKFARAKEFGATECINPQDFSKPIQEVLIEMTDGGVDYSFECIGNVKVMRAALEACHKGWGVSVVVGVAASGEEIATRPFQLVTGRTWKGTAFGGWKSVESVPKLVSEYMSKKIKVDEFVTHNLSFDEINKAFELMHSGKSIRTVVKI Predict reactive species |
| Full Name | alcohol dehydrogenase 5 (class III), chi polypeptide |
| Calculated Molecular Weight | 374 aa, 40 kDa |
| Observed Molecular Weight | 40 kDa |
| GenBank Accession Number | BC014665 |
| Gene Symbol | ADH5 |
| Gene ID (NCBI) | 128 |
| RRID | AB_2881587 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P11766 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
ADH5, also named as ADHX, FDH FALDH GSH-FDH ADH3 and GSNOR, belongs to the zinc-containing alcohol dehydrogenase family and Class-III subfamily. It is remarkably ineffective in oxidizing ethanol, but it readily catalyzes the oxidation of long-chain primary alcohols and the oxidation of S-(hydroxymethyl) glutathione. ADH5 mediates multiple cardiovascular functions. It plays in regulating heterocellular communication in the artery wall. (PMID:21071693). ADH5 immunostaining is distributed in both the nucleus and cytoplasm of the retinal ganglion cells(PMID:22117533).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for GSNOR,ADH5 antibody 66193-1-Ig | Download protocol |
| IHC protocol for GSNOR,ADH5 antibody 66193-1-Ig | Download protocol |
| WB protocol for GSNOR,ADH5 antibody 66193-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
GSNOR antibody (Cat# 66193-1-Ig) has 9 total citations published in European Heart Journal, Redox Biology, Molecular Psychiatry, Arteriosclerosis Thrombosis and Vascular Biology, Alzheimer's & Dementia, European Journal of Pharmacology, Frontiers in Physiology, and FASEB Journal. Research fields span cardiovascular disease, redox biology, neurodegeneration/Alzheimer's disease, vascular remodeling, ferroptosis, intestinal homeostasis, and hepatic one-carbon metabolism.
| Species | Application | Title |
|---|---|---|
Eur Heart J Left bundle branch vs biventricular pacing: mechanistic insights from a canine model. | ||
Redox Biol Loss of erythrocyte arginase-1 impairs vasorelaxation due to endothelial GSNOR overexpression and denitrosylation of G protein subunits. | ||
Redox Biol GSNOR reprograms nitrosylation to drive endothelial-to-mesenchymal transition and fibrotic vascular remodeling. | ||
Mol Psychiatry Degradation of FA reduces Aβ neurotoxicity and Alzheimer-related phenotypes. | ||
Arterioscler Thromb Vasc Biol Arginase-1 Deletion in Erythrocytes Promotes Vascular Calcification via Enhanced GSNOR (S-Nitrosoglutathione Reductase) Expression and NO Signaling in Smooth Muscle Cells | ||
Alzheimers Dement (N Y) New insight into Alzheimer's disease: Light reverses Aβ-obstructed interstitial fluid flow and ameliorates memory decline in APP/PS1 mice. |
Reviews
What customers say
One reviewer rated this antibody 4/5 stars for Western Blot at a 1:8000 dilution, testing it on HepG2, human liver, and mouse liver tissues. They found it to be a good and strong antibody overall but noted that it did not recognize mouse ADH5, despite the datasheet claiming mouse reactivity.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Hua (Verified Customer) (06-17-2021) | Good and strong antibody. However, it doesn't recognize the ADH5 from mouse origin. On the datasheet, it claims that this antibody works with mouse.
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