GSNOR,ADH5 Monoclonal antibody, PBS Only

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  WB analysis of HuH-7 using 66193-1-Ig (same clone as 66193-1-PBS)

  HuH-7 cells were subjected to SDS PAGE followed by western blot with 66193-1-Ig (GSNOR,ADH5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66193-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 66193-1-Ig (same clone as 66193-1-PBS)

  WB result of GSNOR,ADH5 antibody (66193-1-Ig; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GSNOR,ADH5 transfected HepG2 cells. This data was developed using the same antibody clone with 66193-1-PBS in a different storage buffer formulation.

• 

  WB analysis of fetal human brain using 66193-1-Ig (same clone as 66193-1-PBS)

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 66193-1-Ig (GSNOR,ADH5 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66193-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human testis using 66193-1-Ig (same clone as 66193-1-PBS)

  human testis tissue were subjected to SDS PAGE followed by western blot with 66193-1-Ig (GSNOR,ADH5 Antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66193-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 66193-1-Ig (same clone as 66193-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66193-1-Ig (GSNOR,ADH5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66193-1-PBS in a different storage buffer formulation.

• 

  WB analysis of pig liver using 66193-1-Ig (same clone as 66193-1-PBS)

  pig liver tissue were subjected to SDS PAGE followed by western blot with 66193-1-Ig (GSNOR,ADH5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66193-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat brain using 66193-1-Ig (same clone as 66193-1-PBS)

  rat brain tissue were subjected to SDS PAGE followed by western blot with 66193-1-Ig (GSNOR,ADH5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66193-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat liver using 66193-1-Ig (same clone as 66193-1-PBS)

  rat liver tissue were subjected to SDS PAGE followed by western blot with 66193-1-Ig (GSNOR,ADH5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66193-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse testis using 66193-1-Ig (same clone as 66193-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 66193-1-Ig (GSNOR,ADH5 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66193-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human testis using 66193-1-Ig (same clone as 66193-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 66193-1-Ig (GSNOR,ADH5 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 66193-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human testis using 66193-1-Ig (same clone as 66193-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 66193-1-Ig (GSNOR,ADH5 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 66193-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 66193-1-Ig (same clone as 66193-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 66193-1-Ig (GSNOR,ADH5 antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66193-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

GSNOR,ADH5 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 66193-1-PBS

Clone No.5E6E2

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

ADH5, 5E6E2, ADH 3, ADH 5, ADH3

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Product Information

66193-1-PBS targets GSNOR,ADH5 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

• Tested Reactivity: human, mouse, rat, pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag9511

  Product name: Recombinant human GSNOR,ADH5 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-374 aa of BC014665

  Sequence: MANEVIKCKAAVAWEAGKPLSIEEIEVAPPKAHEVRIKIIATAVCHTDAYTLSGADPEGCFPVILGHEGAGIVESVGEGVTKLKAGDTVIPLYIPQCGECKFCLNPKTNLCQKIRVTQGKGLMPDGTSRFTCKGKTILHYMGTSTFSEYTVVADISVAKIDPLAPLDKVCLLGCGISTGYGAAVNTAKLEPGSVCAVFGLGGVGLAVIMGCKVAGASRIIGVDINKDKFARAKEFGATECINPQDFSKPIQEVLIEMTDGGVDYSFECIGNVKVMRAALEACHKGWGVSVVVGVAASGEEIATRPFQLVTGRTWKGTAFGGWKSVESVPKLVSEYMSKKIKVDEFVTHNLSFDEINKAFELMHSGKSIRTVVKI

• Full Name: alcohol dehydrogenase 5 (class III), chi polypeptide
• Calculated Molecular Weight: 374 aa, 40 kDa
• Observed Molecular Weight: 40 kDa
• GenBank Accession Number: BC014665
• Gene Symbol: ADH5
• Gene ID (NCBI): 128
• RRID: AB_2881587
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: P11766
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

ADH5, also named as ADHX, FDH FALDH GSH-FDH ADH3 and GSNOR, belongs to the zinc-containing alcohol dehydrogenase family and Class-III subfamily. It is remarkably ineffective in oxidizing ethanol, but it readily catalyzes the oxidation of long-chain primary alcohols and the oxidation of S-(hydroxymethyl) glutathione. ADH5 mediates multiple cardiovascular functions. It plays in regulating heterocellular communication in the artery wall. (PMID:21071693). ADH5 immunostaining is distributed in both the nucleus and cytoplasm of the retinal ganglion cells(PMID:22117533).