Tested Applications
| Positive WB detected in | hTERT-RPE1 cells, HEK-293 cells, C6 cells, Neuro-2a cells, U-87 MG cells, U-251 cells |
| Positive IHC detected in | human placenta tissue, human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HEK-293 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
66203-1-Ig targets SERPINE2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag1830 Product name: Recombinant human SERPINE2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 28-397 aa of BC015663 Sequence: EELGSNTGIQVFNQIVKSRPHDNIVISPHGIASVLGMLQLGADGRTKKQLAMVMRYGVNGVGKILKKINKAIVSKKNKDIVTVANAVFVKNASEIEVPFVTRNKDVFQCEVRNVNFEDPASACDSINAWVKNETRDMIDNLLSPDLIDGVLTRLVLVNAVYFKGLWKSRFQPENTKKRTFVAADGKSYQVPMLAQLSVFRCGSTSAPNDLWYNFIELPYHGESISMLIALPTESSTPLSAIIPHISTKTIDSWMSIMVPKRVQVILPKFTAVAQTDLKEPLKVLGITDMFDSSKANFAKITTGSENLHVSHILQKAKIEVSEDGTKASAATTAILIARSSPPWFIVDRPFLFFIRHNPTGAVLFMGQINK Predict reactive species |
| Full Name | serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 2 |
| Calculated Molecular Weight | 44 kDa |
| Observed Molecular Weight | 44 kDa |
| GenBank Accession Number | BC015663 |
| Gene Symbol | SERPINE2 |
| Gene ID (NCBI) | 5270 |
| RRID | AB_2881594 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P07093 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
SERPINE2 also called protease nexin-1 or glial-derived neurite promoting factor, is a member of the serine protease inhibitor (SERPIN) superfamily. It would be a potent inhibitor of the urokinase-plasminogen activator (uPA), tissue-type PA (tPA), thrombin, trypsin, factor Xia, and prostasin. SERPINE2 is widely expressed in various tissues, including endothelial cells, fibroblasts, smooth muscle cells, tumor cells, glial cells, neurons, and placental cells.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for SERPINE2 antibody 66203-1-Ig | Download protocol |
| IHC protocol for SERPINE2 antibody 66203-1-Ig | Download protocol |
| WB protocol for SERPINE2 antibody 66203-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The SERPINE2 Monoclonal antibody (1E11F12), catalog number 66203-1-Ig, has 8 total citations published in journals including Advanced Materials, Theranostics, Frontiers in Cell and Developmental Biology, Scientific Reports, Breast Cancer, Animals, Journal of Cardiovascular Development and Disease, and Oncology Letters. The research fields span tumor vasculogenic mimicry, hepatocellular carcinoma, osteosarcoma prognosis, breast cancer, comparative proteomics, pulmonary hypertension, and tumor expression patterns.
| Species | Application | Title |
|---|---|---|
Adv Mater Glycated ECM Derived Carbon Dots Inhibit Tumor Vasculogenic Mimicry by Disrupting RAGE Nuclear Translocation and Its Interaction With HMGB1 | ||
Theranostics Vitamin D binding protein (VDBP) hijacks twist1 to inhibit vasculogenic mimicry in hepatocellular carcinoma | ||
Front Cell Dev Biol BAP31 Promotes Tumor Cell Proliferation by Stabilizing SERPINE2 in Hepatocellular Carcinoma. | ||
Sci Rep Integrating artificial intelligence in osteosarcoma prognosis: the prognostic significance of SERPINE2 and CPT1B biomarkers | ||
Breast Cancer Targeting focal adhesion kinase inhibits cell migration and non-angiogenic vascularization in malignant breast cancer. | ||
Animals (Basel) Comparative Analysis of Proteomic Characteristics in Seminal Plasma Between Horses and Donkeys. |
