Tested Applications
| Positive WB detected in | human peripheral blood platelets, pig liver tissue, pig serum, HuH-7 cells, U-251 cells, SH-SY5Y cells |
| Positive IHC detected in | human kidney tissue, mouse lung tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
66261-1-Ig targets Serpin E1/PAI-1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, pig samples.
| Tested Reactivity | human, mouse, pig |
| Cited Reactivity | human, mouse, rat, pig |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag22411 Product name: Recombinant human SERPINE1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 24-158 aa of BC010860 Sequence: VHHPPSYVAHLASDFGVRVFQQVAQASKDRNVVFSPYGVASVLAMLQLTTGGETQQQIQAAMGFKIDDKGMAPALRHLYKELMGPWNKDEISTTDAIFVQRDLKLVQGFMPHFFRLFRSTVKQVDFSEVERARFI Predict reactive species |
| Full Name | serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1 |
| Calculated Molecular Weight | 402 aa, 45 kDa |
| Observed Molecular Weight | 45-50 kDa |
| GenBank Accession Number | BC010860 |
| Gene Symbol | PAI-1 |
| Gene ID (NCBI) | 5054 |
| RRID | AB_2881648 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P05121 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
SERPINE1, also named Plasminogen activator inhibitor type 1 (PAI-1), is a member of the serine protease inhibitor (SERPIN) superfamily. PAI-1 is the principal inhibitor of tissue plasminogen activator (tPA) and uPA, and hence is an inhibitor of fibrinolysis. Defects in this gene are the cause of plasminogen activator inhibitor-1 deficiency (PAI-1 deficiency), and high concentrations of the gene product are associated with thrombophilia. The mature 379-residue protein had a calculated unglycosylated molecular mass of 42.7 kD. Three putative glycosylation sites were found.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for Serpin E1/PAI-1 antibody 66261-1-Ig | Download protocol |
| IHC protocol for Serpin E1/PAI-1 antibody 66261-1-Ig | Download protocol |
| WB protocol for Serpin E1/PAI-1 antibody 66261-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The Serpin E1/PAI-1 Monoclonal antibody (Cat# 66261-1-Ig) has 50 citations published in journals including Nature Aging, ACS Nano, Redox Biology, Biomaterials, Cardiovascular Research, FASEB Journal, Phytomedicine, and Cell Reports Medicine. Research fields encompass oncology (colorectal, prostate, glioblastoma, cervical, gastric cancer), vascular biology and endothelial dysfunction, fibrosis (pulmonary, renal, adhesion prevention), cellular senescence, diabetes, ARDS, sepsis, stroke, lipid metabolism, and aging. Validated applications include WB, IF, and IHC across human, mouse, rat, and pig models.
| Species | Application | Title |
|---|---|---|
Mol Cancer GP73-dependent regulation of exosome biogenesis promotes colorectal cancer liver metastasis | ||
Nat Aging Single-cell and spatial RNA sequencing identify divergent microenvironments and progression signatures in early- versus late-onset prostate cancer | ||
Nat Aging Extracellular vesicles derived from senescent hepatocytes drive pan-cancer metastasis in aging | ||
ACS Nano Nonswellable Hydrogel Patch with Tissue-Mimetic Mechanical Characteristics Remodeling In Vivo Microenvironment for Effective Adhesion Prevention | ||
Redox Biol SERPINE1 drives ferroptosis in acute respiratory distress syndrome by disrupting mitochondrial NAD(+) homeostasis and suppressing Sirt3 activity. | ||
Adv Sci (Weinh) Rupestonic Acid of Artemisia Rupestris L. Extract Treats Pulmonary Fibrosis in COPD by Targeting TGF-β1. |
Reviews
What customers say
The single review for this product reports a 5-star rating from an immunohistochemistry application. The user worked with paraffin-embedded hamster lung tissue at a 1:100 dilution, using Tris-EDTA buffer (pH 9) for antigen retrieval. Overall, the feedback indicates the antibody performed well for IHC on this tissue type.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Mona (Verified Customer) (10-17-2021) | Paraffin-embedded hamster tissue from the lung. Antigen retrieval was performed with Tris-EDTA buffer (pH 9).
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