CLTB Monoclonal antibody

CLTB Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 66270-1-Ig

Clone No. 1F9C2

Publications (1)

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Host / Isotype

Mouse / IgG1

Reactivity

human, pig

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

Clathrin light chain B, clathrin, light chain (Lcb), CLTB, LCB

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Unconjugated

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Filter: AllWBIHCIF/ICC

WB analysis of pig brain using 66270-1-Ig
pig brain tissue were subjected to SDS PAGE followed by western blot with 66270-1-Ig (CLTB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human liver using 66270-1-Ig
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66270-1-Ig (CLTB Antibody) at dilution of 1:200 (under 10x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human liver using 66270-1-Ig
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66270-1-Ig (CLTB Antibody) at dilution of 1:200 (under 40x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human kidney using 66270-1-Ig
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66270-1-Ig (CLTB Antibody) at dilution of 1:200 (under 10x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human kidney using 66270-1-Ig
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66270-1-Ig (CLTB Antibody) at dilution of 1:200 (under 40x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of HeLa using 66270-1-Ig
Immunofluorescent analysis of (-20℃ Ethanol ) fixed HeLa cells using 66270-1-Ig(CLTB antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Overview

Tested Applications
Product Information
Publications (1)
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in	pig brain tissue
Positive IHC detected in	human liver tissue, human kidney tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66270-1-Ig targets CLTB in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, pig samples.

Tested Reactivity	human, pig
Cited Reactivity	human
Host / Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Immunogen	CatNo: Ag24347 Product name: Recombinant human CLTB protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-211 aa of BC006457 Sequence: MADDFGFFSSSESGAPEAAEEDPAAAFLAQQESEIAGIENDEGFGAPAGSHAAPAQPGPTSGAGSEDMGTTVNGDVFQEANGPADGYAAIAQADRLTQEPESIRKWREEQRKRLQELDAASKVTEQEWREKAKKDLEEWNQRQSEQVEKNKINNRASEEAFVKESKEETPGTEWEKVAQLCDFNPKSSKQCKDVSRLRSVLMSLKQTPLSR Predict reactive species
Full Name	clathrin, light chain (Lcb)
Calculated Molecular Weight	23 kDa
Observed Molecular Weight	32-38 kDa
GenBank Accession Number	BC006457
Gene Symbol	CLTB
Gene ID (NCBI)	1212
RRID	AB_2881655
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein G purification
UNIPROT ID	P09497
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Clathrin is the major protein of the polyhedral coat of coated pits and vesicles which entrap specific macromolecules during receptor-mediated endocytosis. The clathrin molecule has a triskelion shape. Each clathrin triskelion is composed of three identical heavy chains (180 kDa) and three light chains of two types, LCA (CLTA) and LCB (CLTB) (30-40 kDa). The light chain subunits are thought to regulate the formation or disassembly of clathrin coats. (PMID: 2445759; 8374173; 3563513)

Protocols

Product Specific Protocols
IF protocol for CLTB antibody 66270-1-Ig	Download protocol
IHC protocol for CLTB antibody 66270-1-Ig	Download protocol
WB protocol for CLTB antibody 66270-1-Ig	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

What published studies show

The CLTB Monoclonal antibody (1F9C2), catalog number 66270-1-Ig, has a total of 1 citation. It was cited in Theranostics (Impact Factor 14.9), in a study on proteomic analysis of intracellular protein corona of nanoparticles, elucidating nano-trafficking networks and nano-bio interactions. The research field is nanomedicine and nanoparticle biology. The antibody was used for Western blot (WB) with human samples.

Species	Application	Title
human	WB	Theranostics Proteomic analysis of intracellular protein corona of nanoparticles elucidates nano-trafficking network and nano-bio interactions. Authors - Mengmeng Qin View Article