TBL2 Monoclonal antibody

TBL2 Monoclonal Antibody for WB, IHC, ELISA

Cat No. 66307-1-Ig

Clone No. 1D5C2

Publications (1)

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Host / Isotype

Mouse / IgA

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

TBL2, transducin (beta) like 2, Transducin beta like protein 2, WBSCR13, WS betaTRP

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Unconjugated

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Filter: AllWBIHC

WB analysis of MCF-7 using 66307-1-Ig
MCF-7 cells were subjected to SDS PAGE followed by western blot with 66307-1-Ig (TBL2 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of Jurkat using 66307-1-Ig
Jurkat cells were subjected to SDS PAGE followed by western blot with 66307-1-Ig (TBL2 Antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of COLO 320 using 66307-1-Ig
COLO 320 cells were subjected to SDS PAGE followed by western blot with 66307-1-Ig (TBL2 Antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human liver using 66307-1-Ig
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66307-1-Ig (TBL2 Antibody) at dilution of 1:200 (under 40x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human colon using 66307-1-Ig
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66307-1-Ig (TBL2 Antibody) at dilution of 1:200 (under 10x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human colon using 66307-1-Ig
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66307-1-Ig (TBL2 Antibody) at dilution of 1:200 (under 40x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human liver using 66307-1-Ig
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66307-1-Ig (TBL2 Antibody) at dilution of 1:200 (under 10x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Overview

Tested Applications
Product Information
Publications (1)
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in	MCF-7 cells, COLO 320 cells, Jurkat cells
Positive IHC detected in	human liver tissue, human colon tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Mouse monoclonal antibodies of IgA isotype can be detected with "anti-mouse IgG (H+L)" secondary antibodies.

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66307-1-Ig targets TBL2 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human
Host / Isotype	Mouse / IgA
Class	Monoclonal
Type	Antibody
Immunogen	CatNo: Ag24225 Product name: Recombinant human TBL2 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 100-446 aa of BC012938 Sequence: SNGKYLATCADDRTIRIWSTKDFLQREHRSMRANVELDHATLVRFSPDCRAFIVWLANGDTLRVFKMTKREDGGYTFTATPEDFPKKHKAPVIDIGIANTGKFIMTASSDTTVLIWSLKGQVLSTINTNQMNNTHAAVSPCGRFVASCGFTPDVKVWEVCFGKKGEFQEVVRAFELKGHSAAVHSFAFSNDSRRMASVSKDGTWKLWDTDVEYKKKQDPYLLKTGRFEEAAGAAPCRLALSPNAQVLALASGSSIHLYNTRRGEKEECFERVHGECIANLSFDITGRFLASCGDRAVRLFHNTPGHRAMVEEMQGHLKRASNESTRQRLQQQLTQAQVTLGGPGAED Predict reactive species
Full Name	transducin (beta)-like 2
Calculated Molecular Weight	446 aa, 50 kDa
Observed Molecular Weight	50 kDa
GenBank Accession Number	BC012938
Gene Symbol	TBL2
Gene ID (NCBI)	26608
RRID	AB_2881689
Conjugate	Unconjugated
Form	Liquid
Purification Method	Thiophilic affinity chromatograph
UNIPROT ID	Q9Y4P3
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TBL2 belongs to a member of the beta-transducin protein family. This gene is commonly deleted in Williams-Beuren syndrome, a developmental disorder with multi-system manifestations caused by haploinsufficiency for contiguous genes deleted in chromosome region 7q11.23. TBL2 encodes a protein with four putative WD40 repeats, which may be involved in some intracellular signaling pathway.

Protocols

Product Specific Protocols
IHC protocol for TBL2 antibody 66307-1-Ig	Download protocol
WB protocol for TBL2 antibody 66307-1-Ig	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

What published studies show

The TBL2 Monoclonal antibody (1D5C2), catalog number 66307-1-Ig, has 1 total citation. It appears in Advanced Science (2024), a high-impact journal (IF 14.1). The cited study investigates TBL2's role in promoting tumorigenesis via PRMT5/WDR77-mediated AKT activation in breast cancer, placing it in the research fields of oncology and signal transduction. The antibody was used for Western blot and immunohistochemistry in human samples.