Tested Applications
| Positive WB detected in | Raji cells, pig spleen tissue, Ramos cells, Daudi cells |
| Positive IHC-Autostainer detected in | human tonsil tissue |
| Positive IHC detected in | human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | human tonsillitis tissue |
| Positive FC detected in | human PBMCs |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC)-AUTOSTAINER | IHC-AUTOSTAINER : 1:125-1:500 |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| Flow Cytometry (FC) | FC : 0.20 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
66308-1-Ig targets CD27 in WB, IHC, IHC-Autostainer, IF-P, FC, ELISA applications and shows reactivity with human, pig samples.
| Tested Reactivity | human, pig |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag24537 Product name: Recombinant human CD27 protein Source: e coli.-derived, PET30a Tag: 6*His Domain: 1-260 aa of BC012160 Sequence: MARPHPWWLCVLGTLVGLSATPAPKSCPERHYWAQGKLCCQMCEPGTFLVKDCDQHRKAAQCDPCIPGVSFSPDHHTRPHCESCRHCNSGLLVRNCTITANAECACRNGWQCRDKECTECDPLPNPSLTARSSQALSPHPQPTHLPYVSEMLEARTAGHMQTLADFRQLPARTLSTHWPPQRSLCSSDFIRILVIFSGMFLVFTLAGALFLHQRRKYRSNKGESPVEPAEPCRYSCPREEEGSTIPIQEDYRKPEPACSP Predict reactive species |
| Full Name | CD27 molecule |
| Calculated Molecular Weight | 29 kDa |
| Observed Molecular Weight | 50-55 kDa |
| GenBank Accession Number | BC012160 |
| Gene Symbol | CD27 |
| Gene ID (NCBI) | 939 |
| ENSEMBL Gene ID | ENSG00000139193 |
| RRID | AB_2881690 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P26842 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CD27 (also known as TNFRSF7) is a type I glycoprotein expressed on some B cells and the majority of T cells, and is a member of the tumor necrosis factor (TNF) receptor family. CD27 is required for generation and long-term maintenance of T cell immunity (PMID: 11062504). It is a receptor for CD70 (CD27L). Ligation of CD27 by CD70 induces strong ubiquitination of TRAF and the activation of both canonical and non-canonical NF-kappaB pathways, as well as the JNK pathway (PMID: 19426224). CD27 may also play a role in apoptosis through association with SIVA1.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for CD27 antibody 66308-1-Ig | Download protocol |
| IF protocol for CD27 antibody 66308-1-Ig | Download protocol |
| IHC protocol for CD27 antibody 66308-1-Ig | Download protocol |
| WB protocol for CD27 antibody 66308-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The CD27 Monoclonal antibody (1C1G3), catalog number 66308-1-Ig, has 4 total citations. These appear in Nature Communications, Theranostics, NPJ Precision Oncology, and Inflammation Research. The associated research fields include nasopharyngeal carcinoma (tertiary lymphoid structures and immunotherapy response), esophageal squamous cell carcinoma (immunotherapeutic resistance), melanoma (CD27 as a biomarker for immunotherapy response), and IgA nephropathy (circulating inflammatory proteins and kidney function decline).
| Species | Application | Title |
|---|---|---|
Nat Commun Single-cell and spatial transcriptome analyses reveal tertiary lymphoid structures linked to tumour progression and immunotherapy response in nasopharyngeal carcinoma | ||
Theranostics MIF-expressing tumor cells mediate immunotherapeutic resistance in esophageal squamous cell carcinoma. | ||
NPJ Precis Oncol CD27 expression is a clinically accessible biomarker for predicting immunotherapy response in melanoma. | ||
Inflamm Res Multiple circulating inflammatory proteins are associated with pathological lesions and kidney function decline in IgA nephropathy. |
