Tested Applications
| Positive WB detected in | PC-12 cells, human heart tissue, HeLa cells, fetal human brain tissue, COLO 320 cells, Caco-2 cells, pig heart tissue, pig brain tissue, rat brain tissue, mouse brain tissue |
| Positive IHC detected in | human breast cancer tissue, human colon cancer tissue, mouse colon tissue, rat colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A549 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
66338-1-Ig targets MMP3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.
| Tested Reactivity | human, mouse, rat, pig |
| Cited Reactivity | human, mouse, rat, pig, monkey |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag12359 Product name: Recombinant human MMP3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 26-477 aa of BC074869 Sequence: GEDTSMNLVQKYLENYYDLEKDVKQFVRRKDSGPVVKKIREMQKFLGLEVTGKLDSDTLEVMRKPRCGVPDVGHFRTFPGIPKWRKTHLTYRIVNYTPDLPKDAVDSAVEKALKVWEEVTPLTFSRLYEGEADIMISFAVREHGDFYPFDGPGNVLAHAYAPGPGINGDAHFDDDEQWTKDTTGTNLFLVAAHEIGHSLGLFHSANTEALMYPLYHSLTDLTRFRLSQDDINGIQSLYGPPPDSPETPLVPTEPVPPEPGTPANCDPALSFDAVSTLRGEILIFKDRHFWRKSLRKLEPELHLISSFWPSLPSGVDAAYEVTSKDLVFIFKGNQFWAIRGNEVRAGYPRGIHTLGFPPTVRKIDAAISDKEKNKTYFFVEDKYWRFDEKRNSMEPGFPKQIAEDFPGIDSKIDAVFEEFGFFYFFTGSSQLEFDPNAKKVTHTLKSNSWLNC Predict reactive species |
| Full Name | matrix metallopeptidase 3 (stromelysin 1, progelatinase) |
| Calculated Molecular Weight | 477 aa, 54 kDa |
| Observed Molecular Weight | 45-60 kDa |
| GenBank Accession Number | BC074869 |
| Gene Symbol | MMP3 |
| Gene ID (NCBI) | 4314 |
| RRID | AB_2881718 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P08254 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Matrix metalloproteinases (MMPs) play a critically important role in extracellular matrix remodeling and have been implicated in a number of key normal and pathologic processes.These proteases have come to represent important therapeutic and diagnostic targets for the treatment and detection of human cancers. MMP-3 activate procollagenase via two pathways: slow direct activation and rapid activation in conjunction with tissue or plasma proteinases. The pro-MMP3 (60 kDa) and the active MMP3 (47 kDa) can be detected through western blot.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for MMP3 antibody 66338-1-Ig | Download protocol |
| IHC protocol for MMP3 antibody 66338-1-Ig | Download protocol |
| WB protocol for MMP3 antibody 66338-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The MMP3 Monoclonal antibody (Cat# 66338-1-Ig) has 88 citations. These appear in journals including Nature Aging, Nature Communications, Biomaterials, Advanced Science, Theranostics, Journal of Nanobiotechnology, Phytomedicine, Frontiers in Pharmacology, International Journal of Molecular Sciences, and Journal of Cell and Molecular Medicine, among others. Research fields span osteoarthritis, rheumatoid arthritis, intervertebral disc degeneration, fibrosis, aging, and cancer, with applications mainly in WB, IHC, and IF.
| Species | Application | Title |
|---|---|---|
Mil Med Res Downregulation of FTO aggravates osteoarthritis with obesity by erasing m(6)A methylation of PDP2. | ||
Nat Aging Comparative analysis of senolytic drugs reveals mitochondrial determinants of efficacy and resistance. | ||
Nat Commun SLC1A5 prevents aortic aneurysm and dissection by glutaminolytic-epigenetic orchestration of vascular smooth muscle cell homeostasis. | ||
Nat Commun The natural flavonoid dihydromyricetin targets senescent cells via PRDX2 and alleviates age-related diseases | ||
J Nanobiotechnology Quercetin nanoformulation-embedded hydrogel inhibits osteopontin mediated ferroptosis for intervertebral disc degeneration alleviation |
