Tested Applications
| Positive WB detected in | human spleen tissue, RAW 264.7 cells, pig spleen tissue, rat spleen tissue |
| Positive IHC detected in | human colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
66456-1-Ig targets Lysozyme in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat, pig samples.
| Tested Reactivity | human, mouse, rat, pig |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag6917 Product name: Recombinant human Lysozyme protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 24-148 aa of BC004147 Sequence: CELARTLKRLGMDGYRGISLANWMCLAKWESGYNTRATNYNAGDRSTDYGIFQINSRYWCNDGKTPGAVNACHLSCSALLQDNIADAVACAKRVVRDPQGIRAWVAWRNRCQNRDVRQYVQGCGV Predict reactive species |
| Full Name | lysozyme (renal amyloidosis) |
| Calculated Molecular Weight | 17 kDa |
| Observed Molecular Weight | 15-17 kDa |
| GenBank Accession Number | BC004147 |
| Gene Symbol | Lysozyme |
| Gene ID (NCBI) | 4069 |
| RRID | AB_2881825 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P61626 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
LYZ(Lysozyme C) is also named as LZM and belongs to the glycosyl hydrolase 22 family. It catalyzes the hydrolysis of the bacterial cell wall beta(1-4) glycosidic linkages between N-acetylmuramic acid and N-acetylglucosamine. In tissues and body fluids this protein is associated with the monocyte-macrophage system and enhance the activity of immunoagents. LYZ is capable of both hydrolysis and transglycosylation; it shows also a slight esterase activity. Defects in LYZ are a cause of amyloidosis type 8 (AMYL8). The full length protein has a signal peptide with 18 amino acid.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for Lysozyme antibody 66456-1-Ig | Download protocol |
| WB protocol for Lysozyme antibody 66456-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
Lysozyme Monoclonal antibody (2G5A8), catalog number 66456-1-Ig, has 6 total citations. These appear in Biomaterials Research, Clinical & Translational Medicine, Cell Reports, Scientific Reports, Journal of Pharmacological Sciences, and Research Square. Associated research fields include cancer cell fate and biomaterials, nasopharyngeal carcinoma tumour microenvironment, vascular inflammation and pyroptosis, bone development via GPR109A/myeloid cells, and NSAID-associated gut mucosal injury with microbiota regulation.
| Species | Application | Title |
|---|---|---|
Clin Transl Med Single-cell transcriptome analysis reveals evolving tumour microenvironment induced by immunochemotherapy in nasopharyngeal carcinoma | ||
Cell Rep Sox10 escalates vascular inflammation by mediating vascular smooth muscle cell transdifferentiation and pyroptosis in neointimal hyperplasia | ||
Sci Rep Sex dependent effects of GPR109A gene deletion in myeloid cells on bone development in mice | ||
J Pharmacol Sci Oxymatrine alleviates NSAID-associated small bowel mucosal injury by regulating MIP-1/CCR1 signalling and gut microbiota | ||
Res Sq Sex-dependent effect of GPR109A gene deletion in myeloid cells on bone development in mice |
