Tested Applications
| Positive WB detected in | HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells |
| Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:150-1:600 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
66460-1-Ig targets IKBKG in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag13358 Product name: Recombinant human IKBKG protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 120-419 aa of BC012114 Sequence: QALREVEHLKRCQQQMAEDKASVKAQVTSLLGELQESQSRLEAATKECQALEGRARAASEQARQLESEREALQQQHSVQVDQLRMQGQSVEAALRMERQAASEEKRKLAQLQVAYHQLFQEYDNHIKSSVVGSERKRGMQLEDLKQQLQQAEEALVAKQEVIDKLKEEAEQHKIVMETVPVLKAQADIYKADFQAERQAREKLAEKKELLQEQLEQLQREYSKLKASCQESARIEDMRKRHVEVSQAPLPPAPAYLSSPLALPSQRRSPPEEPPDFCCPKCQYQAPDMDTLQIHVMECIE Predict reactive species |
| Full Name | inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase gamma |
| Calculated Molecular Weight | 48 kDa |
| Observed Molecular Weight | 48 kDa |
| GenBank Accession Number | BC012114 |
| Gene Symbol | IKBKG |
| Gene ID (NCBI) | 8517 |
| RRID | AB_2881829 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q9Y6K9 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
IKBKG, also named as FIP3, NEMO, IKKAP1 and IKKG, is specifically phosphorylate serine or threonine residues that are followed by a proline residue. IKBKG is regulatory subunit of the IKK core complex which phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. Its binding to scaffolding polyubiquitin seems to play a role in IKK activation by multiple signaling receptor pathways.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for IKBKG antibody 66460-1-Ig | Download protocol |
| IHC protocol for IKBKG antibody 66460-1-Ig | Download protocol |
| WB protocol for IKBKG antibody 66460-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The IKBKG Monoclonal antibody (1F2E2), catalog number 66460-1-Ig, has 1 total citation. It was used in a Western blot study published in Virology Sinica (2023), investigating how the Classical swine fever virus NS5A protein antagonizes the innate immune response by inhibiting NF-κB signaling. The research falls within the fields of virology, innate immunity, and NF-κB signaling pathway biology.
| Species | Application | Title |
|---|---|---|
Virol Sin Classical swine fever virus NS5A protein antagonizes innate immune response by inhibiting the NF-κB signaling |
Reviews
What customers say
The single review rates this antibody 4 out of 5 stars at a 1:1000 dilution for Western Blot. The reviewer confirmed strong performance in WB using human U2OS and HeLa cells, with validation in IKBKG/NEMO knockout cells generated by CRISPR. Additionally, the antibody was reported to work well in immunofluorescence on methanol-fixed cells, also validated in KO cells. Overall, the feedback highlights reliable specificity and functional validation across two applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Emmanuel (Verified Customer) (11-28-2025) | Good antibody for western blot. Validated using IKBKG/NEMO knockout cells generated by CRISPR (see picture) Works also in immunofluorescence in Methanol fixed cells (also validated in KO cells).
|
