CoraLite® Plus 488-conjugated IKBKG Monoclonal antibody

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  IF Staining of HeLa using CL488-66460

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 IKBKG antibody (CL488-66460, Clone: 1F2E2 ) at dilution of 1:200.

IKBKG Monoclonal Antibody for IF/ICC

Cat No. CL488-66460

Clone No.1F2E2

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

IF/ICC

Synonyms

AMCBX1, FIP3, FIP-3, Fip3p, I-kappa-B kinase subunit gamma

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Tested Applications

• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66460 targets IKBKG in IF/ICC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag13358

  Product name: Recombinant human IKBKG protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 120-419 aa of BC012114

  Sequence: QALREVEHLKRCQQQMAEDKASVKAQVTSLLGELQESQSRLEAATKECQALEGRARAASEQARQLESEREALQQQHSVQVDQLRMQGQSVEAALRMERQAASEEKRKLAQLQVAYHQLFQEYDNHIKSSVVGSERKRGMQLEDLKQQLQQAEEALVAKQEVIDKLKEEAEQHKIVMETVPVLKAQADIYKADFQAERQAREKLAEKKELLQEQLEQLQREYSKLKASCQESARIEDMRKRHVEVSQAPLPPAPAYLSSPLALPSQRRSPPEEPPDFCCPKCQYQAPDMDTLQIHVMECIE

• Full Name: inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase gamma
• Calculated Molecular Weight: 48 kDa
• Observed Molecular Weight: 48 kDa
• GenBank Accession Number: BC012114
• Gene Symbol: IKBKG
• Gene ID (NCBI): 8517
• RRID: AB_2919330
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: Q9Y6K9
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

IKBKG, also named as FIP3, NEMO, IKKAP1 and IKKG, is specifically phosphorylate serine or threonine residues that are followed by a proline residue. IKBKG is regulatory subunit of the IKK core complex which phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. Its binding to scaffolding polyubiquitin seems to play a role in IKK activation by multiple signaling receptor pathways.