Filter: AllWBIHCIF-PAffinity

WB analysis using 66501-1-Ig
Various lysates were subjected to SDS PAGE followed by western blot with 66501-1-Ig (PCCB antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis using 66501-1-Ig
Various lysates were subjected to SDS PAGE followed by western blot with 66501-1-Ig (PCCB antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 66501-1-Ig
WB result of PCCB antibody (66501-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PCCB transfected HepG2 cells.
IHC staining of human heart using 66501-1-Ig
Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66501-1-Ig (PCCB antibody) at dilution of 1:300 (under 10x lens).
IHC staining of human heart using 66501-1-Ig
Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66501-1-Ig (PCCB antibody) at dilution of 1:300 (under 40x lens).
IF Staining of mouse kidney using 66501-1-Ig
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse kidney tissue using PCCB antibody (66501-1-Ig, Clone: 1A9A5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of human kidney using 66501-1-Ig
Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using 66501-1-Ig (PCCB antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).
Affinity and Kinetic Characterization of 66501-1-Ig
Biolayer interferometry (BLl) kinetic assay of 66501-1-Ig against Human PCCB was performed. The affinity constant is 2.99 nM.

Featured Product
KD/KO Validated

PCCB Monoclonal antibody

PCCB Monoclonal Antibody for WB, IHC, IF-P, ELISA

Cat No. 66501-1-Ig

Clone No.1A9A5

Review Product

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF-P, ELISA

Synonyms

1A9A5, EC:6.4.1.3, PCCase subunit beta, Propanoyl-CoA:carbon dioxide ligase subunit beta, Propionyl-CoA carboxylase beta chain, mitochondrial

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Unconjugated

CoraLite®488 CoraLite®594

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information
Protocols

Print datasheet

MSDS SDS

Tested Applications

Positive WB detected in	Jurkat cells, human heart tissue, HepG2 cells, HSC-T6 cells, pig heart tissue, rat heart tissue, mouse liver tissue
Positive IHC detected in	human heart tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF-P detected in	mouse kidney tissue, human kidney tissue

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:5000-1:10000
Immunohistochemistry (IHC)	IHC : 1:150-1:600
Immunofluorescence (IF)-P	IF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66501-1-Ig targets PCCB in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity	human, mouse, rat, pig
Host / Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Immunogen	CatNo: Ag1608 Product name: Recombinant human PCCB protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 240-539 aa of BC013768 Sequence: TQEELGGAKTHTTMSGVAHRAFENDVDALCNLRDFFNYLPLSSQDPAPVRECHDPSDRLVPELDTIVPLESTKAYNMVDIIHSVVDEREFFEIMPNYAKNIIVGFARMNGRTVGIVGNQPKVASGCLDINSSVKGARFVRFCDAFNIPLITFVDVPGFLPGTAQEYGGIIRHGAKLLYAFAEATVPKVTVITRKAYGGAYDVMSSKHLCGDTNYAWPTAEIAVMGAKGAVEIIFKGHENVEAAQAEYIEKFANPFPAAVRGFVDDIIQPSSTRARICCDLDVLASKKVQRPWRKHANIPL Predict reactive species
Full Name	propionyl Coenzyme A carboxylase, beta polypeptide
Calculated Molecular Weight	58 kDa
Observed Molecular Weight	58 kDa
GenBank Accession Number	BC013768
Gene Symbol	PCCB
Gene ID (NCBI)	5096
RRID	AB_2881865
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein G purification
UNIPROT ID	P05166
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IF protocol for PCCB antibody 66501-1-Ig	Download protocol
IHC protocol for PCCB antibody 66501-1-Ig	Download protocol
WB protocol for PCCB antibody 66501-1-Ig	Download protocol