Filter: AllWBIHCIF/ICC

WB analysis using 66529-1-Ig
Untreated and PNGase F-treated lysates of THP-1 cells and Mo7e cells were subjected to SDS PAGE followed by western blot with 66529-1-Ig (FCGR2A / CD32a antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB analysis of U-937 using 66529-1-Ig
Various lysates were subjected to SDS PAGE followed by western blot with 66529-1-Ig (FCGR2A / CD32a antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB analysis of Raji using 66529-1-Ig
Raji cells were subjected to SDS PAGE followed by western blot with 66529-1-Ig (FCGR2A / CD32a antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
IHC staining of human liver cancer using 66529-1-Ig
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66529-1-Ig (FCGR2A / CD32a antibody) at dilution of 1:5000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 66529-1-Ig
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66529-1-Ig (FCGR2A / CD32a antibody) at dilution of 1:5000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human tonsillitis using 66529-1-Ig
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66529-1-Ig (FCGR2A / CD32a antibody) at dilution of 1:5000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human tonsillitis using 66529-1-Ig
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66529-1-Ig (FCGR2A / CD32a antibody) at dilution of 1:5000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of THP-1 using 66529-1-Ig
Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using FCGR2A / CD32a antibody (66529-1-Ig, Clone: 1D3F1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

FCGR2A / CD32a Monoclonal antibody

Name: FCGR2A / CD32a Monoclonal antibody
Brand: Proteintech
SKU: 66529-1-Ig

FCGR2A / CD32a Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 66529-1-Ig

Clone No.1D3F1

Review Product

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

CD32, 1D3F1, CD32A, Fc gamma RII a, Fc gamma RIIa

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information
Protocols

Print datasheet

MSDS SDS

Tested Applications

Positive WB detected in	TF-1 cells, Raji cells, U-937 cells, Mo7e cells, K-562 cells, THP-1 cells
Positive IHC detected in	human liver cancer tissue, human tonsillitis tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	THP-1 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:5000-1:50000
Immunohistochemistry (IHC)	IHC : 1:2500-1:10000
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66529-1-Ig targets FCGR2A / CD32a in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Host / Isotype	Mouse / IgG2b
Class	Monoclonal
Type	Antibody
Immunogen	CatNo: Ag25878 Product name: Recombinant human FCGR2A protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 44-215 aa of BC020823 Sequence: LEPPWINVLQEDSVTLTCQGARSPESDSIQWFHNGNLIPTHTQPSYRFKANNNDSGEYTCQTGQTSLSDPVHLTVLSEWLVLQTPHLEFQEGETIMLRCHSWKDKPLVKVTFFQNGKSQKFSHLDPTFSIPQANHSHSGDYHCTGNIGYTLFSSKPVTITVQVPSMGSSSPM Predict reactive species
Full Name	Fc fragment of IgG, low affinity IIa, receptor (CD32)
Calculated Molecular Weight	35 kDa
Observed Molecular Weight	42 kDa
GenBank Accession Number	BC020823
Gene Symbol	CD32a
Gene ID (NCBI)	2212
RRID	AB_2881892
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	P12318
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IgG Fc receptors (FcγRs) play important roles in immune responses. Low affinity IgG Fc receptor Fc gamma RIIa (FCGR2A, CD32a) is a cell surface receptor found on phagocytic cells such as macrophages and neutrophils, and is involved in the process of phagocytosis and clearing of immune complexes.

Protocols

Product Specific Protocols
IF protocol for FCGR2A / CD32a antibody 66529-1-Ig	Download protocol
IHC protocol for FCGR2A / CD32a antibody 66529-1-Ig	Download protocol
WB protocol for FCGR2A / CD32a antibody 66529-1-Ig	Download protocol