CoraLite® Plus 488-conjugated FCGR2A / CD32a Monoclonal antibody

FCGR2A / CD32a Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

IF

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1D3F1

Cat no : CL488-66529

Synonyms

CD32, CD32A, CDw32, Fc gamma RII a, Fc gamma RIIa, FCG2, FcGR, FCGR2, FCGR2A, FCGR2A / CD32a, FCGR2A1, FcRII a, IGFR2, IgG Fc receptor II a

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Validation Data Gallery

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  IF Staining of THP-1 using CL488-66529

  Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using CoraLite® Plus 488 FCGR2A / CD32a antibody (CL488-66529, Clone: 1D3F1 ) at dilution of 1:50.

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  IF Staining of human tonsillitis using CL488-66529

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CL488-66529 (FCGR2A / CD32a antibody) at dilution of 1:50.

• 

  IF Staining of human tonsillitis using CL488-66529

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CL488-66529 (FCGR2A / CD32a antibody) at dilution of 1:50.

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Tested Applications

• Positive IF detected in: THP-1 cells, human tonsillitis tissue

Recommended dilution

• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66529 targets FCGR2A / CD32a in IF applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: FCGR2A / CD32a fusion protein Ag25878
• Full Name: Fc fragment of IgG, low affinity IIa, receptor (CD32)
• Calculated Molecular Weight: 35 kDa
• GenBank Accession Number: BC020823
• Gene Symbol: FCGR2A
• Gene ID (NCBI): 2212
• RRID: AB_2883350
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IgG Fc receptors (FcγRs) play important roles in immune responses. Low affinity IgG Fc receptor Fc gamma RIIa (FCGR2A, CD32a) is a cell surface receptor found on phagocytic cells such as macrophages and neutrophils, and is involved in the process of phagocytosis and clearing of immune complexes. This antibody is conjugated with CL488, Ex/Em 488 nm/515 nm.