CoraLite®488-conjugated FCGR2A / CD32a Monoclonal antibody

FCGR2A / CD32a Monoclonal Antibody for FC, IF, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

IF, FC, ELISA

Conjugate

CoraLite®488 Fluorescent Dye

CloneNo.

1D3F1

Cat no : CL488-66529

Synonyms

CD32, CD32A, CDw32, Fc gamma RII a, Fc gamma RIIa, FCG2, FcGR, FCGR2, FCGR2A, FCGR2A1, FcRII a, IGFR2, IgG Fc receptor II a



Tested Applications

Positive IF detected inhuman tonsillitis tissue
Positive FC detected inK526
For other applications, we recommend the unconjugated version of this antibody, 66529-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

CL488-66529 targets FCGR2A / CD32a in IF, FC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen FCGR2A / CD32a fusion protein Ag25878
Full Name Fc fragment of IgG, low affinity IIa, receptor (CD32)
Calculated molecular weight 35 kDa
GenBank accession numberBC020823
Gene symbol FCGR2A
Gene ID (NCBI) 2212
Conjugate CoraLite®488 Fluorescent Dye
Excitation/Emission maxima wavelengths488 nm/515 nm
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

IgG Fc receptors (FcγRs) play important roles in immune responses. Low affinity IgG Fc receptor Fc gamma RIIa (FCGR2A, CD32a) is a cell surface receptor found on phagocytic cells such as macrophages and neutrophils, and is involved in the process of phagocytosis and clearing of immune complexes. This antibody is conjugated with CL488, Ex/Em 488 nm/515 nm.

Protocols

Product Specific Protocols
IF protocol for CL488-conjugated FCGR2A / CD32a antibody CL488-66529Download protocol
FC protocol for CL488-conjugated FCGR2A / CD32a antibody CL488-66529Download protocol
Standard Protocols
Click here to view our Standard Protocols