Filter: AllWBCytometric bead array sample testCytometric bead array

WB analysis using 66571-1-Ig (same clone as 66571-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 66571-1-Ig (CIN85 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control. This data was developed using the same antibody clone with 66571-1-PBS in a different storage buffer formulation.
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Various lysates were subjected to SDS PAGE followed by western blot with 66571-1-Ig (CIN85 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control. This data was developed using the same antibody clone with 66571-1-PBS in a different storage buffer formulation.
WB analysis of K-562 using 66571-1-Ig (same clone as 66571-1-PBS)
K-562 cells were subjected to SDS PAGE followed by western blot with 66571-1-Ig (CIN85 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66571-1-PBS in a different storage buffer formulation.
WB analysis of PC-12 using 66571-1-Ig (same clone as 66571-1-PBS)
PC-12 cells were subjected to SDS PAGE followed by western blot with 66571-1-Ig (CIN85 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66571-1-PBS in a different storage buffer formulation.
WB analysis of rat brain using 66571-1-Ig (same clone as 66571-1-PBS)
rat brain and mouse brain tissue were subjected to SDS PAGE followed by western blot with 66571-1-Ig (CIN85 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66571-1-PBS in a different storage buffer formulation.
Cytometric bead array sample test of MP51571-1
Cytometric bead array sample test of MP51571-1, CIN85 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66571-1-PBS. Detection antibody: 66571-2-PBS.
Cytometric bead array standard curve of MP51571-1
Cytometric bead array standard curve of MP51571-1, CIN85 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66571-1-PBS. Detection antibody: 66571-2-PBS. Standard:Ag2777. Range: 0.391-50 ng/mL.

CIN85 Monoclonal antibody, PBS Only (Capture)

Name: CIN85 Monoclonal antibody, PBS Only (Capture)
Brand: Proteintech
SKU: 66571-1-PBS

CIN85 Monoclonal Antibody for WB, Cytometric bead array, Indirect ELISA

Cat No. 66571-1-PBS

Clone No.3C12D2

Review Product

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, Cytometric bead array, Indirect ELISA

Synonyms

SH3KBP1, 3C12D2, Cbl-interacting protein of 85 kDa, CD2 binding protein 3, CD2-binding protein 3

Formulation: PBS Only

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

Print datasheet

MSDS SDS

Product Information

66571-1-PBS targets CIN85 as part of a matched antibody pair:

MP51571-1: 66571-1-PBS capture and 66571-2-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity	human, mouse, rat
Host / Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Immunogen	CatNo: Ag2777 Product name: Recombinant human CIN85 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-328 aa of BC015806 Sequence: MVEAIVEFDYQAQHDDELTISVGEIITNIRKEDGGWWEGQINGRRGLFPDNFVREIKKEMKKDPLTNKAPEKPLHEVPSGNSLLSSETILRTNKRGERRRRRCQVAFSYLPQNDDELELKVGDIIEVVGEVEEGWWEGVLNGKTGMFPSNFIKELSGESDELGISQDEQLSKSSLRETTGSESDGGDSSSTKSEGANGTVATAAIQPKKVKGVGFGDIFKDKPIKLRPRSIEVENDFLPVEKTIGKKLPATTATPDSSKTEMDSRTKSKDYCKVIFPYEAQNDDELTIKEGDIVTLINKDCIDVGWWEGELNGRRGVFPDNFVKLLPP Predict reactive species
Full Name	SH3-domain kinase binding protein 1
Calculated Molecular Weight	665 aa, 73 kDa
Observed Molecular Weight	85 kDa
GenBank Accession Number	BC015806
Gene Symbol	SH3KBP1/CIN85
Gene ID (NCBI)	30011
RRID	AB_2881932
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein G purification
UNIPROT ID	Q96B97
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

CIN85 is identified as a Cbl-interacting protein of 85 kDa and belongs to adaptor/scaffold proteins. It composed of three N-terminal SH3 domains followed by a proline-rich region and a C-terminal coiled-coil region. In association with c-Cbl (Casitas B-lineage lymphoma), an E3 ubiquitin ligase. As an adaptor protein, CIN85 is involved in Cbl-dependent EGFR internalization, intracellular receptor trafficking, sorting and degradation. Moreover, through the SH3 domains and the proline-rich region, CIN85 is implicated in many protein-protein interactions and it is found to play important roles in other processes such as apoptosis, rearrangement of actin cytoskeleton, cell adhesion, immunological synapse, cell migration and invasion.