CIN85 Monoclonal antibody

CIN85 Monoclonal Antibody for WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB,ELISA

Conjugate

Unconjugated

CloneNo.

3C12D2

Cat no : 66571-1-Ig

Synonyms

CD2 binding protein 3, CD2BP3, CIN85, GIG10, HSB 1, MIG18, SH3KBP1



Tested Applications

Positive WB detected inHL-60 cells, K-562 cells, rat brain tissue, mouse brain tissue, THP-1 cells, Jurkat cells, Ramos cells, Raji cells, Daudi cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Sample-dependent, check data in validation data gallery

Product Information

66571-1-Ig targets CIN85 in WB,ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CIN85 fusion protein Ag2777
Full Name SH3-domain kinase binding protein 1
Calculated molecular weight 665 aa, 73 kDa
Observed molecular weight 85 kDa
GenBank accession numberBC015806
Gene symbol SH3KBP1
Gene ID (NCBI) 30011
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CIN85 is identified as a Cbl-interacting protein of 85 kDa and belongs to adaptor/scaffold proteins. It composed of three N-terminal SH3 domains followed by a proline-rich region and a C-terminal coiled-coil region. In association with c-Cbl (Casitas B-lineage lymphoma), an E3 ubiquitin ligase. As an adaptor protein, CIN85 is involved in Cbl-dependent EGFR internalization, intracellular receptor trafficking, sorting and degradation. Moreover, through the SH3 domains and the proline-rich region, CIN85 is implicated in many protein-protein interactions and it is found to play important roles in other processes such as apoptosis, rearrangement of actin cytoskeleton, cell adhesion, immunological synapse, cell migration and invasion.

Protocols

Product Specific Protocols
WB protocol for CIN85 antibody 66571-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols